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Western Blot (WB) (Western BlotPositive WB detected in: A375 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysateAll lanes: FARP1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 119 KDaObserved band size: 119 KDa)

Rabbit anti-Human FARP1 Polyclonal Antibody | anti-FARP1 antibody

FARP1 Antibody

Gene Names
FARP1; CDEP; PLEKHC2; PPP1R75; FARP1-IT1
Reactivity
Human
Applications
ELISA
Purity
>95%,Protein G purified
Synonyms
FARP1; Polyclonal Antibody; FARP1 Antibody; FERM; RhoGEF and pleckstrin domain-containing protein 1; Chondrocyte-derived ezrin-like protein; Pleckstrin homology domain-containing family C member 2; PH domain-containing family C member 2; CDEP; PLEKHC2; anti-FARP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid
Sequence Length
129
Applicable Applications for anti-FARP1 antibody
ELISA (EIA)
Immunogen
Recombinant human FERM, RhoGEF and pleckstrin domain-containing protein 1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-74927 / sc-74929 / sc-98722
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in: A375 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysateAll lanes: FARP1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 119 KDaObserved band size: 119 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: A375 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysateAll lanes: FARP1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 119 KDaObserved band size: 119 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7002691 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7002691 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7002691 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using MBS7002691 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS7002691 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7002691 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-FARP1 antibody
Functions as guanine nucleotide exchange factor for RAC1. May play a role in semaphorin signaling. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,138 Da
NCBI Official Full Name
FERM, RhoGEF and pleckstrin domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
FERM, ARH/RhoGEF and pleckstrin domain protein 1
NCBI Official Symbol
FARP1
NCBI Official Synonym Symbols
CDEP; PLEKHC2; PPP1R75; FARP1-IT1
NCBI Protein Information
FERM, RhoGEF and pleckstrin domain-containing protein 1
UniProt Protein Name
FERM, RhoGEF and pleckstrin domain-containing protein 1
UniProt Gene Name
FARP1
UniProt Synonym Gene Names
CDEP; PLEKHC2; PH domain-containing family C member 2
UniProt Entry Name
FARP1_HUMAN

NCBI Description

This gene encodes a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Uniprot Description

FARP1: May function as Rho-guanine nucleotide exchange factor. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q32.2

Cellular Component: cell junction; cytoplasm; cytoskeleton; cytosol; dendrite; dendritic spine; extrinsic to internal side of plasma membrane; filopodium

Molecular Function: cytoskeletal protein binding; Rac guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: dendrite morphogenesis; positive regulation of GTPase activity; regulation of Rho protein signal transduction; synaptogenesis

Research Articles on FARP1

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Product Notes

The FARP1 farp1 (Catalog #AAA7002691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FARP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FARP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the FARP1 farp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FARP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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