Rabbit anti-Human POLR2D Polyclonal Antibody | anti-POLR2D antibody

POLR2D Antibody; HRP conjugated

Cat. Num. AAA7000406

• Gene Names: POLR2D; RBP4; RPB16; HSRBP4; HSRPB4
• Reactivity: Human
• Applications: ELISA
• Purity: >95%,Protein G purified
• Synonyms: POLR2D; Polyclonal Antibody; POLR2D Antibody; HRP conjugated; DNA-directed RNA polymerase II subunit RPB4; RNA polymerase II subunit B4; DNA-directed RNA polymerase II subunit D; RNA polymerase II 16 kDa subunit; RPB16; anti-POLR2D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 142

• Applicable Applications for anti-POLR2D antibody: ELISA (EIA)
• Immunogen: Recombinant human DNA-directed RNA polymerase II subunit RPB4 protein
• Conjugation: HRP
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Related Product Information for anti-POLR2D antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 4758574
• NCBI GeneID: 5433
• NCBI Accession #: NP_004796.1
• NCBI GenBank Nucleotide #: NM_004805.3
• UniProt Accession #: O15514; Q52LT4
• Molecular Weight: 16,311 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB4
• NCBI Official Synonym Full Names: polymerase (RNA) II subunit D
• NCBI Official Symbol: POLR2D
• NCBI Official Synonym Symbols: RBP4; RPB16; HSRBP4; HSRPB4
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB4
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB4
• UniProt Gene Name: POLR2D
• UniProt Synonym Gene Names: RNA polymerase II subunit B4; RPB16
• UniProt Entry Name: RPB4_HUMAN

NCBI Description

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2D: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Belongs to the eukaryotic RPB4 RNA polymerase subunit family.

Protein type: EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Transcription initiation complex; Transferase; Nucleotide Metabolism - purine; RNA processing

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus

Molecular Function: DNA-directed RNA polymerase activity; nucleotide binding; single-stranded DNA binding; single-stranded RNA binding; translation initiation factor binding

Biological Process: DNA repair; gene expression; mRNA capping; mRNA catabolic process, deadenylation-dependent decay; mRNA export from nucleus during heat stress; nuclear mRNA splicing, via spliceosome; nucleotide-excision repair; positive regulation of translational initiation; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA splicing; RNA-mediated gene silencing; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; viral reproduction