Nanog-TAT Active Protein | NANOG active protein

Human Nanog-TAT

Cat. Num. AAA691909

• Reactivity: Human
• Purity: 95% (SDS-PAGE, HPLC)
• Synonyms: Nanog-TAT; Human Nanog-TAT; NANOG active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: 95% (SDS-PAGE, HPLC)
• Form/Format: Lyophilized
• Sequence: MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMP HTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK V PVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYK QVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YH QGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCT QS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEG LNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR
• Sequence Length: 305

• Label/Conjugation: TAT-Fusion protein
• Biological Activity: Testing in progress.

Related Product Information for NANOG active protein

Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.2 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide.

Product Categories/Family for NANOG active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 153945816
• NCBI GeneID: 79923
• NCBI Accession #: NP_079141.2
• NCBI GenBank Nucleotide #: NM_024865.2
• UniProt Accession #: Q9H9S0; Q2TTG0; Q6JZS5; D3DUU4
• Molecular Weight: 34,620 Da
• NCBI Official Full Name: homeobox protein NANOG
• NCBI Official Synonym Full Names: Nanog homeobox
• NCBI Official Symbol: NANOG
• NCBI Protein Information: homeobox protein NANOG; hNanog; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
• UniProt Protein Name: Homeobox protein NANOG
• Protein Family: Homeobox protein
• UniProt Gene Name: NANOG
• UniProt Synonym Gene Names: hNanog
• UniProt Entry Name: NANOG_HUMAN

NCBI Description

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. Ref.3 Ref.8 Ref.11

Subunit structure: Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281

By similarity.

Subcellular location: Nucleus Ref.11.

Tissue specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Ref.1 Ref.2 Ref.3 Ref.6 Ref.9

Developmental stage: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. Ref.3 Ref.7 Ref.9 Ref.10 Ref.11

Miscellaneous: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.

Sequence similarities: Belongs to the Nanog homeobox family.Contains 1 homeobox DNA-binding domain.