PAI-2 active protein

Human PAI-2

Cat. Num. AAA691745

• Gene Names: SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Synonyms: PAI-2; Human PAI-2; Plasminogen Activator Inhibitor-2; Urokinase Inhibitor; PAI-2 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGS RGST EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY P DAILQAQAA DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASF REE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IP NLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQ RT PVQMMYLREK LNIGYIEDLK AQILELPYAG DVSMFLLLPD EIA DVSTGLE LLESEITYDK LNKWTSKDKM AEDEVEVYIP QFKLEEHYE L RSILRSMGME DAFNKGRANF SGMSERNDLF LSEVFHQAMV DVNE EGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH KITNCILFFG RFSSP
• Sequence Length: 415

• Biological Activity: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 degree C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was obtained at a concentration of 1.0 ug/ml

Related Product Information for PAI-2 active protein

PAI-2 is an inhibitory serpin expressed mainly in keratinocytes, activated monocytes, and placental trophoblasts. It exists predominantly as a 47 kDa nonglycosylated intracellular protein which can be induced to be secreted as 60 kDa glycoprotein. The glycosylated and unglycosylated forms of PAI-2 are equally effective as inhibitors of urokinase-type plasminogen activator (uPA), the only established physiological target of this serpin. PAI-2 has a unique ability to form dormant polymers spontaneously and reversibly under physiological conditions. The physiological relevance of this property, which is neither a consequence of any mutation in the PAI-2 gene nor associated with any known disorder, is still unclear. However, it appears that the formation of intracellular dormant polymers may be important for the controlled release of the inhibitor from PAI-2 producing cells. Plasma levels of PAI-2 are usually low or undetectable, except during pregnancy and in some forms of monocytic leukemia. Secretion of PAI-2 from the placenta normally occurs during the third trimester of pregnancy and accounts for the dramatic increase in PAI-2 levels (up to 250 ng/ml), which are maintained at these levels until postpartum, and then rapidly decline. In addition to its vital role in protecting the placenta from degradation by uPA and/or uPA-activated proteases, PAI-2 has been shown to be essential for the prevention of metastatic spread of neck, lung and breast cancers. The beneficial effect of PAI-2 seen in these studies is presumed to stem from its ability to inhibit uPA-dependent cell dissemination. PAI-2 has also been reported to inhibit keratinocyte proliferation, and to participate in the innate immune response during viral infection. Recombinant human PAI-2 is a 415-residue nonglycosylated protein.

Product Categories/Family for PAI-2 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 219689110
• NCBI GeneID: 5055
• NCBI Accession #: NP_001137290.1
• NCBI GenBank Nucleotide #: NM_001143818.1
• UniProt Accession #: P05120; Q96E96
• Molecular Weight: 46,596 Da
• NCBI Official Full Name: plasminogen activator inhibitor 2
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade B (ovalbumin), member 2
• NCBI Official Symbol: SERPINB2
• NCBI Official Synonym Symbols: PAI; PAI2; PAI-2; PLANH2; HsT1201
• NCBI Protein Information: plasminogen activator inhibitor 2; serpin B2; monocyte Arg-serpin; urokinase inhibitor; placental plasminogen activator inhibitor; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2
• UniProt Protein Name: Plasminogen activator inhibitor 2
• UniProt Gene Name: SERPINB2
• UniProt Synonym Gene Names: PAI2; PLANH2; PAI-2
• UniProt Entry Name: PAI2_HUMAN

Uniprot Description

Function: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

Subunit structure: Interacts with PSMB1. Ref.9

Subcellular location: Cytoplasm. Secreted › extracellular space.

Post-translational modification: The signal sequence is not cleaved.

Sequence similarities: Belongs to the serpin family. Ov-serpin subfamily.