PEDF active protein

Human PEDF

Cat. Num. AAA691710

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Reactivity: Human
• Purity: 90 % (SDS-PAGE, HPLC)
• Synonyms: PEDF; Human PEDF; Recombinant Human PEDF; PEDF active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: 90 % (SDS-PAGE, HPLC)
• Form/Format: Lyophilized
• Sequence: MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYD LYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL Y YDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFV APL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IP DEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSD PK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEE SLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSL F DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHL TFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRG
• Sequence Length: 418

• Endotoxin Level: < 0.1 ng per ug of PEDF
• Biological Activity: Data not available.

Related Product Information for PEDF active protein

PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity (1) should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers. Recombinant PEDF is a 44.5 kDa non-glycosylated protein containing 400 amino acid residues. (1) Notari, I. et al. J Biol Chem., Vol. 281, 38022-38037.

Product Categories/Family for PEDF active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NM_002615.5
• UniProt Accession #: P36955; Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092
• Molecular Weight: 44.5 kDa
• NCBI Official Full Name: pigment epithelium-derived factor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• UniProt Protein Name: Pigment epithelium-derived factor
• UniProt Gene Name: SERPINF1
• UniProt Synonym Gene Names: PEDF; PEDF
• UniProt Entry Name: PEDF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

Uniprot Description

Function: Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Ref.13 Ref.14

Subcellular location: Secreted. Melanosome. Note: Enriched in stage I melanosomes. Ref.17

Tissue specificity: Retinal pigment epithelial cells and blood plasma. Ref.11

Developmental stage: Expressed in quiescent cells.

Domain: The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.

Post-translational modification: The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Ref.19 Ref.23

Involvement in disease: Osteogenesis imperfecta 6 (OI6) [MIM:613982]: A form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI6 is a severe, autosomal recessive form compatible with OI type III in the Sillence classification.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.21

Sequence similarities: Belongs to the serpin family.

Sequence caution: The sequence AAA84914.1 differs from that shown. Reason: Frameshift at position 356. The sequence AAA93524.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.