Western Blot (WB)
Rabbit anti-Human TRYPSIN Monoclonal Antibody | anti-TRYPSIN antibody
Anti - TRYPSIN
Western Blot (WB)
Western Blot (WB)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. Ref.9
Catalytic activity: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor: Binds 1 calcium ion per subunit.
Subcellular location: Secreted › extracellular space.
Post-translational modification: Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
Involvement in disease: Pancreatitis (PCTT) [MIM:167800]: A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8 Ref.11 Ref.12 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23
Sequence similarities: Belongs to the peptidase S1 family.Contains 1 peptidase S1 domain.
Caution: Tyr-154 was proposed to be phosphorylated (Ref.15) but it has been shown (Ref.14) to be sulfated instead. Phosphate and sulfate groups are similar in mass and size, and this can lead to erroneous interpretation of the results.
Mass spectrometry: Molecular mass is 24348±2 Da from positions 24 - 247. Determined by ESI. Ref.15
Research Articles on TRYPSIN
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Product Notes
The TRYPSIN prss1 (Catalog #AAA684164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - TRYPSIN reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRYPSIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). WB - 1:2 000, ELISA - 1:10 000 - 1:20 000. Researchers should empirically determine the suitability of the TRYPSIN prss1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRYPSIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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