Mouse Sigma S Monoclonal Antibody | anti-Sigma S antibody

mAb to Sigma S

Cat. Num. AAA680013

• Gene Names: rpoS; abrD; appR; csi2; dpeB; ECK2736; JW5437; katF; nur; otsX; sigS
• Reactivity: Most enterics
• Applications: Western Blot, ELISA
• Synonyms: Sigma S; Monoclonal Antibody; mAb to Sigma S; MAb to sigmaS (sigma S) subunit of E.coli RNA polymerase; E. coli RNA Sigma S Monoclonal Antibody; Sigma 38; rpoS; appR; katF; nur; otsX; sigS; anti-Sigma S antibody

• Host: Mouse
• Reactivity: Most enterics
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1RS1
• Specificity: Sigma S (E. coli Sigma S (Strain MG1655))
• Form/Format: Ascites
• Sequence Positions: 33-256
• Sequence Length: 330

• Applicable Applications for anti-Sigma S antibody: Western Blot (WB), ELISA (EIA), Transcription Inhibition
• Application Notes: Transcription Inhibition - Initiation: Yes; Western Blot: Yes - dilute at 1:1000
• Immunogen: Recombinant Protein
• Reported Reactivity: E Coli, Klebsiella, Salmonella, Serratia, Bordetella and an unidentified protein at 60 kDA
• Cross Reactivity Note: E Coli, Klebsiella, Salmonella, Serratia, Bordetella and an unidentified protein at 60 kDA
• Polyol-responsive: No
• Reconstitution: Add 100ul of ddH2O to the tube containing antibody and mix to dissolve
• Product Note: Does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthrobacter, or Borrelia *Cross reactivity and Applications data is per published literature and/or unpublished data.
• Preparation and Storage: Store at -20 degree C

Related Product Information for anti-Sigma S antibody

MAb to sigma S subunit of E.coli RNA polymerase. Sigma S is a 38 kDA subunit of the E.coli RNA polymerase.
Sigma S is a 38kDa transcription factor of E. coli RNA Polymerase
sigmaS (sigma S) is a 38kDa subunit of the E.coli RNA polymerase

Sigma transcription factor controlling a regulation of genes required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon and other genes by modifying the affinity of RNA polymerase for the promoters.

NCBI and Uniprot Product Information

• NCBI GI #: 16130648
• NCBI GeneID: 947210
• NCBI Accession #: NP_417221
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P13445; Q2MA88; Q79EB7
• Molecular Weight: 37,972 Da
• NCBI Official Full Name: RNA polymerase, sigma S (sigma 38) factor
• NCBI Official Symbol: rpoS
• NCBI Official Synonym Symbols: abrD; appR; csi2; dpeB; ECK2736; JW5437; katF; nur; otsX; sigS
• NCBI Protein Information: RNA polymerase, sigma S (sigma 38) factor
• UniProt Protein Name: RNA polymerase sigma factor RpoS
• UniProt Gene Name: rpoS
• UniProt Synonym Gene Names: appR; katF; nur; otsX; sigS
• UniProt Entry Name: RPOS_ECOLI

NCBI Description

Polyamine modulon. [More information is available at EcoGene: EG10510]. rpoS encodes the alternative sigma factor S, a subunit of RNA polymerase that acts as the master regulator of the general stress response in Escherichia coli. [More information is available at EcoCyc: EG10510].

Uniprot Description

Function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.

Subunit structure: Interacts with the RNA polymerase core enzyme and RssB.

Subcellular location: Cytoplasm

Potential.

Induction: Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease.

Sequence similarities: Belongs to the sigma-70 factor family. RpoS subfamily.

Sequence caution: The sequence BAE76818.1 differs from that shown. Reason: Erroneous termination at position 33. Translated as Gln.The sequence CAA34435.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAA78692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.