Goat Anti-Mouse IgG2b (gamma 2b chain specific) Secondary Antibody
Goat Anti-Mouse IgG2b, Human ads-APC
Reactivity
Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins.
Applications
ELISA, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity chromatography on mouse IgG2b covalently linked to agarose
Synonyms
Goat Anti-Mouse IgG2b (gamma 2b chain specific); Polyclonal Secondary Antibody; Goat Anti-Mouse IgG2b; Human ads-APC; Human ads - Allophycocyanin; Goat Anti-Mouse IgG2b (gamma 2b chain specific) secondary antibody
Host
Pooled antisera from goates hyperimmunized with mouse IgG2b paraproteins
Reactivity
Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins.
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy chain of mouse IgG2b as demonstrated by ELISA, FLISA, and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Purity/Purification
Affinity chromatography on mouse IgG2b covalently linked to agarose
Form/Format
Allophycocyanin (APC) Conjugate
Applicable Applications for Goat Anti-Mouse IgG2b (gamma 2b chain specific) secondary antibody
ELISA; FLISA; Immunoblotting; Indirect Immunostaining in conjunction with mouse IgG2b monoclonal antibodies
Application Notes
WORKING DILUTIONS
Immunofluorescence: APC conjugates: <= 0.1 ug/10^6 cells
Immunofluorescence: APC conjugates: <= 0.1 ug/10^6 cells
Source
Pooled antisera from goats hyperimmunized with mouse IgG2b paraproteins.
Cross Absorption
Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% 50 mM Tris, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% 50 mM Tris, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Product Categories/Family for Goat Anti-Mouse IgG2b (gamma 2b chain specific) secondary antibody
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Product Notes
The Goat Anti-Mouse IgG2b (gamma 2b chain specific) (Catalog #AAA674499) is a Secondary Antibody produced from Pooled antisera from goates hyperimmunized with mouse IgG2b paraproteins and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Mouse IgG2b, Human ads-APC reacts with Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Goat Anti-Mouse IgG2b (gamma 2b chain specific) can be used in a range of immunoassay formats including, but not limited to, ELISA; FLISA; Immunoblotting; Indirect Immunostaining in conjunction with mouse IgG2b monoclonal antibodies. WORKING DILUTIONS Immunofluorescence: APC conjugates: <= 0.1 ug/10^6 cells. Researchers should empirically determine the suitability of the Goat Anti-Mouse IgG2b (gamma 2b chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Mouse IgG2b (gamma 2b chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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