Rat anti-Mouse CD94 Monoclonal Antibody | anti-KLRD1 antibody
Rat Anti-Mouse CD94-FITC
Gene Names
KLRD1; CD94
Reactivity
Mouse
Applications
Flow Cytometry
Synonyms
CD94; Monoclonal Antibody; Rat Anti-Mouse CD94-FITC; Rat Anti-Mouse CD94 - FITC; Rat Anti-Mouse CD94; anti-KLRD1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
18d3
Specificity
Mouse CD94 receptor subunit, Mr 70 kDa
The monoclonal antibody 18D3 reacts with the mouse CD94 receptor subunit, a 70kDa type II transmembrane glycoprotein. CD94 is expressed on the surface of NK cells, NK T cells and some memory phenotype CD8+ cells.2 When CD94 is paired with NKG2A, C, or E subunit, it binds the nonclassical MHC class Ib molecule Qa-1b and results in a CD94high population by flow cytometry.1, 2 Approximately half of NK cells do not express NKG2A, C or E; these cells express CD94 on the surface in a form that does not bind Qa-1b and is believed to be nonfunctional, producing a CD94low population by flow cytometry.1, 2 CD94 is thought to play a role in the adhesion and activation of the NK cell lineage.
The monoclonal antibody 18D3 reacts with the mouse CD94 receptor subunit, a 70kDa type II transmembrane glycoprotein. CD94 is expressed on the surface of NK cells, NK T cells and some memory phenotype CD8+ cells.2 When CD94 is paired with NKG2A, C, or E subunit, it binds the nonclassical MHC class Ib molecule Qa-1b and results in a CD94high population by flow cytometry.1, 2 Approximately half of NK cells do not express NKG2A, C or E; these cells express CD94 on the surface in a form that does not bind Qa-1b and is believed to be nonfunctional, producing a CD94low population by flow cytometry.1, 2 CD94 is thought to play a role in the adhesion and activation of the NK cell lineage.
Form/Format
Fluorescein (FITC) Conjugate
Sequence Length
54
Applicable Applications for anti-KLRD1 antibody
Flow Cytometry
Application Notes
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Immunogen
CHO cells transfected with the B6 allele of CD94
Characterization
To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Vance, R.E., A.M. Jamieson, and D.H. Raulet. 1999. J. Exp. Med. 190:1801.
2. Liu, S., and D.H. Raulet. Personal communication.
2. Liu, S., and D.H. Raulet. Personal communication.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,513 Da
NCBI Official Full Name
CD94
NCBI Official Synonym Full Names
killer cell lectin-like receptor subfamily D, member 1
NCBI Official Symbol
KLRD1
NCBI Official Synonym Symbols
CD94
NCBI Protein Information
natural killer cells antigen CD94; KP43; CD94 antigen; NK cell receptor; OTTHUMP00000238754; OTTHUMP00000238755; OTTHUMP00000238756; OTTHUMP00000238758; OTTHUMP00000239093
UniProt Protein Name
Natural killer cells antigen CD94
Protein Family
UniProt Gene Name
KLRD1
UniProt Synonym Gene Names
CD94
UniProt Entry Name
KLRD1_HUMAN
NCBI Description
Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
Function: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
Subunit structure: Can form disulfide-bonded heterodimer with NKG2 family members. Ref.8 Ref.9 Ref.10
Subcellular location: Membrane; Single-pass type II membrane protein.
Tissue specificity: Natural killer cells.
Sequence similarities: Contains 1 C-type lectin domain.
Research Articles on KLRD1
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Product Notes
The KLRD1 klrd1 (Catalog #AAA673582) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD94-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD94 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry. Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the KLRD1 klrd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD94, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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