Rat anti-Mouse CD38 Monoclonal Antibody | anti-CD38 antibody

Rat Anti-Mouse CD38-FITC

Cat. Num. AAA673338

• Gene Names: CD38; T10
• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD38; Monoclonal Antibody; Rat Anti-Mouse CD38-FITC; Rat Anti-Mouse CD38 - FITC; Rat Anti-Mouse CD38; anti-CD38 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone Number: NIMR-5
• Specificity: CD38, Mr 42-46 kDa; Murine CD38, a type II transmembrane glycoprotein, is a bifunctional ectoenzyme capable of catabolizing nicotinamide adenine dinucleotide (NAD+) to cyclic ADP-ribose (cADPR), and then hydrolyzing cADPR to adenosine diphosphoribose (ADPR). It is expressed at high levels on the surface of peripheral B-lineage cells, and at low density on germinal center B cells from unimmunized mice. It has also been reported to be expressed at moderate levels on NK cells, a proportion of peripheral T cells, and a subpopulation of thymocytes which are mostly TCR alpha beta ^+, CD4^-, CD8^-. Murine CD38 is also expressed by all Mac-1^+ macrophages in the peritoneal cavities of unimmunized mice, but not by unstimulated bone-marrow-derived macrophages. Monoclonal antibodies to CD38 have been shown to induce B- and T-cell proliferation, protect B cells from apoptosis, and inhibit B lymphopoiesis.^1-8
• Form/Format: Fluorescein (FITC) Conjugate
• Sequence Length: 300

• Applicable Applications for anti-CD38 antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): Induction of B lymphocyte proliferation; ; Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. Harada, N., L. Santos-Argumedo, R. Chang, J. C. Grimaldi, F. E. Lund, C. I. Brannan, N. G. Copeland, N. A. Jenkins, A. W. Heath, and R. M. Parkhouse. 1993. J. Immunol. 151:3111.
2. Santos-Argumedo, L., C. Teixeira, G. Preece, P. A. Kirkham, and R. M. E. Parkhouse. 1993. J. Immunol. 151:3119.
3. Howard, M., J. C. Grimaldi, J. F. Bazan, F. E. Lund, L. Santos-Argumedo, R. M. E. Parkhouse, T. F. Walseth, and H. C. Lee. 1993. Science 262:1056.
4. Lund, F., N. Solvason, J. C. Grimaldi, R. M. Parkhouse, and M. Howard. 1995. Immunol. Today 16:469.
5. Kirkham, P. A., L. Santos-Argumedo, M. M. Harnett, and R. M. Parkhouse. 1994. Immunology 83:513.
6. Bean, A. G. D., D. I. Godfrey, W. G. Ferlin, L. Santos-Argumedo, R. M. E. Parkhouse, M. C. Howard, and A. Zlotnik. 1996. Int. Immunol. 7:213.
7. Oliver, A. and J. Kearney. 1996. Personal Communication.
8. Kumagai, M., E. Coustan-Smith, D. J. Murray, O. Silvennoinen, K. G. Murti, W. E. Evans, F. Malavasi, and D. Campana. 1995. J. Exp. Med. 181:1101.

NCBI and Uniprot Product Information

• NCBI GI #: 1911103
• NCBI GeneID: 952
• UniProt Accession #: P28907; O00121; O00122; Q96HY4; Q4FCX6
• Molecular Weight: 34,328 Da
• NCBI Official Full Name: CD38
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: T10
• NCBI Protein Information: ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 antigen (p45); OTTHUMP00000158633; OTTHUMP00000217743; NAD(+) nucleosidase; cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Protein Name: ADP-ribosyl cyclase 1
• Protein Family: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Gene Name: CD38
• UniProt Entry Name: CD38_HUMAN

NCBI Description

CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq]

Uniprot Description

Function: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.

Catalytic activity: NAD+ + H2O = ADP-ribose + nicotinamide.

Enzyme regulation: ATP inhibits the hydrolyzing activity.

Subcellular location: Membrane; Single-pass type II membrane protein.

Tissue specificity: Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. Ref.2

Developmental stage: Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.

Sequence similarities: Belongs to the ADP-ribosyl cyclase family.