Rat anti-Mouse CD4/L3T4 Monoclonal Antibody | anti-Cd4 antibody

Rat Anti-Mouse CD4/L3T4-PE

Cat. Num. AAA673158

• Gene Names: Cd4; L3T4; Ly-4
• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD4/L3T4; Monoclonal Antibody; Rat Anti-Mouse CD4/L3T4-PE; Rat Anti-Mouse CD4/L3T4 - Phycoerythrin-Labeled; Rat Anti-Mouse CD4/L3T4; anti-Cd4 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat (Lewis) IgG2b kappa
• Clone Number: GK1.5
• Specificity: CD4/L3T4a, Mr 52 kDa; CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested.^1,2 CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. ^3-6 The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. ^1,2 In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4^+ cells.^7,8 The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.^1,8-13
• Form/Format: R - phycoerythrin (R - PE) Conjugate
• Sequence Length: 457

• Applicable Applications for anti-Cd4 antibody: Flow Cytometry; Immunohistochemistry; Immunoprecipitation
• Application Notes: Research Application(s): In vivo and in vitro depletion of CD4^+ cells; Inhibition of T helper function; Fractionation of CD4^+ cells; ; Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
• Immunogen: Cloned mouse CTL line V4
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this package insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (RPE- CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5 Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29.
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933.
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473.
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller-Sieburg. 1992. Blood 60:1717.
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71.
6. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1.
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445.
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118.
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808.
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5.
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131.
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207.
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1.
15. Jia, W. and J. F. Kearney. 1996. Personal communication.

NCBI and Uniprot Product Information

• NCBI GI #: 24659577
• NCBI GeneID: 12504
• UniProt Accession #: P06332; O55054; P70443; Q3TSV7; Q61396
• Molecular Weight: 51,297 Da
• NCBI Official Full Name: CD4 antigen
• NCBI Official Synonym Full Names: CD4 antigen
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: L3T4; Ly-4
• NCBI Protein Information: T-cell surface glycoprotein CD4; OTTMUSP00000027399; T-cell surface antigen T4/Leu-3; T-cell differentiation antigen L3T4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: Cd4
• UniProt Entry Name: CD4_MOUSE

Uniprot Description

Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subunit structure: Associates with p56-LCK. Interacts with SPG21

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.