Rat anti-Mouse IFN- gamma Monoclonal Antibody | anti-IFNG antibody

Rat Anti-Mouse IFN-g-UNLB

Cat. Num. AAA670101

• Gene Names: IFNG; IFG; IFI
• Reactivity: Mouse
• Applications: ELISA, Flow Cytometry, Immunofluorescence, Western Blot
• Synonyms: IFN- gamma; Monoclonal Antibody; Rat Anti-Mouse IFN-g-UNLB; Rat Anti-Mouse IFN-g - Purified; Unlabeled Antibody; Rat Anti-Mouse IFN- gamma; anti-IFNG antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: XMG1.2
• Specificity: Mouse Interferon- gamma (IFN- gamma)1-3 This is a neutralizing antibody
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 166

• Applicable Applications for anti-IFNG antibody: ELISA; Flow Cytometry; Immunofluorescence; Western Blot
• Application Notes: Research Application(s): ELISA Detection: XMG1.2 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IFN- gamma protein levels. Biotinylated XMG1.2 antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IFN- gamma as the standard.; Western Blotting: The purified XMG1.2 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IFN- gamma under reducing conditions.; Immunofluorescence/Flow Cytometry: XMG1.2 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN- gamma -positive cells within mixed cell populations.4-6 Recombinant mouse IFN- gamma or Rat IgG1 should be used as a specificity control. FITC conjugated XMG1.2 antibodies are useful for intracellular staining in cells producing IFN- gamma. our intracellular staining buffers are recommended for this application.; Neutralization: The LE/AF form of XMG1.2 can be used for neutralizing mouse IFN- gamma bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
• Immunogen: E. coli-expressed recombinant mouse IFN- gamma
• Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Cherwinski, H.M., J.H. Schumacher, K.D. Brown, and T.R. Mossman. 1987. J. Exp. Med. 166:1229.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
3. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.
4. Vickingson, A., K. Pederson, and D. Muller. 1994. J. Immunol. Meth. 173:219.
5. Ferrick, D.A., M.D. Schrenzel, T. Mulvania, B. Hsieh, W.G. Ferlin, and H. Lepper. 1995. Nature 373:255.
6. Hsieh, B., M. Schrenzel, T. Mulvania, H. Lepper, L. DiMolfetto-Landon, and D. Ferrick. 1996. J. Immunol. 156:232.

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.2
• UniProt Accession #: P01579; Q53ZV4; B5BU88; Q14609; Q14610; Q14611; Q14612; Q14613; Q14614; Q14615; Q8NHY9; Q96LA2
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon, gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma; IFN-gamma; immune interferon; OTTHUMP00000240111
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: IFNG
• UniProt Entry Name: IFNG_HUMAN

NCBI Description

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.

Uniprot Description

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Released primarily from activated T lymphocytes.

Post-translational modification: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

Involvement in disease: In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [

MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

Pharmaceutical use: Available under the name Actimmune (Genentech). Used for reducing the frequency and severity of serious infections associated with chronic granulomatous disease (CGD).

Sequence similarities: Belongs to the type II (or gamma) interferon family.