Rat anti-Mouse IL-4 Monoclonal Antibody | anti-IL4 antibody
Rat Anti-Mouse IL-4-UNLB
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Synonyms
IL-4; Monoclonal Antibody; Rat Anti-Mouse IL-4-UNLB; Rat Anti-Mouse IL-4 - Purified; Unlabeled Antibody; Rat Anti-Mouse IL-4; anti-IL4 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
BVD4-1D11
Specificity
Mouse Interleukin-4 (IL-4)1, 2 (This is a neutralizing antibody.)
Form/Format
Purified (UNLB) Antibody
Sequence Length
133
Applicable Applications for anti-IL4 antibody
ELISA; Flow Cytometry; Immunofluorescence; Western Blot
Application Notes
Research Application(s): ELISA Capture: BVD4-1D11 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified BVD4-1D11 antibody should be paired with biotinylated BVD6-24G2 antibody as the detection antibody, with purified recombinant mouse IL-4 as the standard.
Immunofluorescence/Flow Cytometry: BVD4-1D11 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control.
Western Blotting: The purified BVD4-1D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-4 under reducing conditions.
Neutralization: The LE/AF form of BVD4-1D11 can be used for neutralizing mouse IL-4 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunofluorescence/Flow Cytometry: BVD4-1D11 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control.
Western Blotting: The purified BVD4-1D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-4 under reducing conditions.
Neutralization: The LE/AF form of BVD4-1D11 can be used for neutralizing mouse IL-4 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunogen
E. coli-expressed recombinant mouse IL-4
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
References
1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
3. Finkelman, F., K. Madden, S. Morris, J. Holmes, N. Bolani,, I. Jkatona, and C. Maliszewski. 1993. Anti-cytokine antibodies as carrier proteins. J. Immunol. 151:1235-1244.
4. Litton, M., B. Sander, E. Murphy, A. O'Garra, and J. Abrams. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J. Immunol. Meth. 175:47-58.
2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
3. Finkelman, F., K. Madden, S. Morris, J. Holmes, N. Bolani,, I. Jkatona, and C. Maliszewski. 1993. Anti-cytokine antibodies as carrier proteins. J. Immunol. 151:1235-1244.
4. Litton, M., B. Sander, E. Murphy, A. O'Garra, and J. Abrams. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J. Immunol. Meth. 175:47-58.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,023 Da
NCBI Official Full Name
interleukin-4
NCBI Official Symbol
IL4
NCBI Protein Information
interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
UniProt Protein Name
Interleukin-4
Protein Family
UniProt Gene Name
IL4
UniProt Entry Name
IL4_PIG
Uniprot Description
Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-4/IL-13 family.
Research Articles on IL4
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Product Notes
The IL4 il4 (Catalog #AAA670066) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IL-4-UNLB reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-4 can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry; Immunofluorescence; Western Blot. Research Application(s): ELISA Capture: BVD4-1D11 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified BVD4-1D11 antibody should be paired with biotinylated BVD6-24G2 antibody as the detection antibody, with purified recombinant mouse IL-4 as the standard. Immunofluorescence/Flow Cytometry: BVD4-1D11 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blotting: The purified BVD4-1D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-4 under reducing conditions. Neutralization: The LE/AF form of BVD4-1D11 can be used for neutralizing mouse IL-4 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the IL4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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