Rat anti-Human IL-10 Monoclonal Antibody | anti-IL-10 antibody
Rat Anti-Human IL-10-BIOT
Reactivity
Human
Applications
ELISA, Western Blot
Synonyms
IL-10; Monoclonal Antibody; Rat Anti-Human IL-10-BIOT; Rat Anti-Human IL-10 - Biotin; Rat Anti-Human/Viral IL-10; anti-IL-10 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
JES3-12G8
Specificity
Human and Viral Interleukin-10 (IL-10)1-4 (This is a neutralizing antibody.)
Form/Format
Biotin (BIOT) Conjugate
Sequence Length
168
Applicable Applications for anti-IL-10 antibody
ELISA; Western Blot
Application Notes
Research Application(s): ELISA Detection: Biotinylated JES3-12G8 monoclonal antibody is the preferred detection antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-4 Biotinylated JES3-12G8 antibody should be paired with purified JES3-9D7 antibody as the capture antibody, with purified recombinant human IL-10 as the standard.
Western Blotting: The purified JES3-12G8 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-10 under reducing conditions. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Western Blotting: The purified JES3-12G8 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-10 under reducing conditions. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunogen
COS-expressed human IL-101
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
References
1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Gotlieb, W.H., J.S. Abrams, J.M. Watson, T. Velu, J.S. Berek, and O. Martinez-Maza. 1992. Presence of IL-10 in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine 4:385-390.
3. Yssel, H., R. de Waal Malefyt, M-G. Roncarolo, J. Abrams, R. Lahesmaa, H. Spits, and J. de Vries. 1992. Interleukin 10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells. J. Immunol. 149:2378-2384.
4. Burdin, N., C. Peronne, J. Banchereau, and F. Rousset. 1993. Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10. J. Exp. Med. 177:295-304.
2. Gotlieb, W.H., J.S. Abrams, J.M. Watson, T. Velu, J.S. Berek, and O. Martinez-Maza. 1992. Presence of IL-10 in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine 4:385-390.
3. Yssel, H., R. de Waal Malefyt, M-G. Roncarolo, J. Abrams, R. Lahesmaa, H. Spits, and J. de Vries. 1992. Interleukin 10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells. J. Immunol. 149:2378-2384.
4. Burdin, N., C. Peronne, J. Banchereau, and F. Rousset. 1993. Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10. J. Exp. Med. 177:295-304.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IL-10
NCBI Official Symbol
SPPV_03
NCBI Protein Information
IL-10
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Product Notes
The IL-10 (Catalog #AAA670023) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Human IL-10-BIOT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-10 can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. Research Application(s): ELISA Detection: Biotinylated JES3-12G8 monoclonal antibody is the preferred detection antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-4 Biotinylated JES3-12G8 antibody should be paired with purified JES3-9D7 antibody as the capture antibody, with purified recombinant human IL-10 as the standard. Western Blotting: The purified JES3-12G8 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-10 under reducing conditions. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the IL-10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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