Flow Cytometry (FC/FACS)
(Fig: 1- Cell surface FLOW analysis of hCD25 in PHA treated human PBMC using 0.5 ug/10^6 cells. Green represents FITC conjugated mouse IgG2a isotype control; red represents anti-hCD25 antibody (10-4125-F).)
Mouse anti-Human hCD25 Monoclonal Antibody | anti-hCD25 antibody
Monoclonal Antibody to hCD25 (Clone: 7G7B6) FITC Conjugated
Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
hCD25; Monoclonal Antibody; Monoclonal Antibody to hCD25 (Clone: 7G7B6) FITC Conjugated; IL2RA; CD25; TAC antigen; p55; anti-hCD25 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
7G7B6
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
0.2 mg/ml in Tris buffer containing 0.05% Azide
Sequence Length
272
Applicable Applications for anti-hCD25 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS analysis: 0.5-1 ug/10^6 cells
Antigen
hCD25
Immunogen Information
Recombinant human CD25 protein was used as an imunogen for this antidody.
Conjugation
FITC
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
Flow Cytometry (FC/FACS)
(Fig: 1- Cell surface FLOW analysis of hCD25 in PHA treated human PBMC using 0.5 ug/10^6 cells. Green represents FITC conjugated mouse IgG2a isotype control; red represents anti-hCD25 antibody (10-4125-F).)
Flow Cytometry (FC/FACS)
(Fig: 1- Cell surface FLOW analysis of hCD25 in PHA treated human PBMC using 0.5 ug/10^6 cells. Green represents FITC conjugated mouse IgG2a isotype control; red represents anti-hCD25 antibody (10-4125-F).)
Related Product Information for anti-hCD25 antibody
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. Though CD25 has been shown to influence effector function of lymphocytes, it is thought to play a greater role in immune tolerance in mice. Constitutive expression of CD25 on Treg cells is thought to be crucial to their survival and maintenance of immune homeostasis. Expression of CD25 and its role in immunology may be species dependent, since CD25 appears to play a larger role in T-cell effector responses in humans compared to mice, and may be somewhat dispensable for the maintenance of Treg cells as seen in patients treated with CD25-blocking antibodies.
Product Categories/Family for anti-hCD25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,819 Da
NCBI Official Full Name
interleukin-2 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 2 receptor subunit alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
NCBI Protein Information
interleukin-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Gene Name
IL2RA
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
NCBI Description
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Uniprot Description
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Research Articles on hCD25
Similar Products
Product Notes
The hCD25 il2ra (Catalog #AAA668991) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to hCD25 (Clone: 7G7B6) FITC Conjugated reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's hCD25 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS analysis: 0.5-1 ug/10^6 cells. Researchers should empirically determine the suitability of the hCD25 il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hCD25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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