Western Blot (WB)
(Fig-1: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 2 ug/ml on Molt-4, Kato III and HepG2 lysates.)
Mouse anti-Human Caspase-8 Monoclonal Antibody | anti-CASP8 antibody
Monoclonal Antibody to Caspase-8 (Clone: ABM14C1)
Protein G Chromatography
Protein G Chromatography
FC/FACS: 0.5-1 ug/10^6
IHC: 5-10 ug/ml
For long-term storage, store at -20 degree C.
Avoid repeat freezing and thawing
Western Blot (WB)
(Fig-1: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 2 ug/ml on Molt-4, Kato III and HepG2 lysates.)
Western Blot (WB)
(Fig-1: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 2 ug/ml on Molt-4, Kato III and HepG2 lysates.)
Flow Cytometry (FC/FACS)
(Fig-2: Intracellular Flow analysis of Caspase-8 antibody in Hela cells using 0.5 ug/ 10^6 cells of anti-Caspase-8 antibody (ABM14C1). Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS)
(Fig-2: Intracellular Flow analysis of Caspase-8 antibody in Hela cells using 0.5 ug/ 10^6 cells of anti-Caspase-8 antibody (ABM14C1). Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS)
(Fig-3: Intracellular Flow analysis of Caspase-8 antibody in Jurkat cells using 0.5 ug/ 10^6 cells of anti-Caspase-8 antibody (ABM14C1). Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS)
(Fig-3: Intracellular Flow analysis of Caspase-8 antibody in Jurkat cells using 0.5 ug/ 10^6 cells of anti-Caspase-8 antibody (ABM14C1). Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Immunohistochemistry (IHC)
(Fig-4: Immunohistochemical analysis of Caspase-8 in human Esophagus using anti-Caspase-8 antibody (Clone: ABM14C1) at 5 ug/ml.)
Immunohistochemistry (IHC)
(Fig-4: Immunohistochemical analysis of Caspase-8 in human Esophagus using anti-Caspase-8 antibody (Clone: ABM14C1) at 5 ug/ml.)
Immunohistochemistry (IHC)
(Fig-5: Immunohistochemical analysis of Caspase-8 in human Tonsil using anti-Caspase-8 antibody (Clone: ABM14C1) at 5 ug/ml.)
Immunohistochemistry (IHC)
(Fig-5: Immunohistochemical analysis of Caspase-8 in human Tonsil using anti-Caspase-8 antibody (Clone: ABM14C1) at 5 ug/ml.)
Western Blot (WB)
(Figure-6: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 4 ug/ml on EL-4 lysate.)
Western Blot (WB)
(Figure-6: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 4 ug/ml on EL-4 lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Uniprot Description
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Research Articles on CASP8
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Product Notes
The CASP8 casp8 (Catalog #AAA668940) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Caspase-8 (Clone: ABM14C1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS). WB: 4-6 ug/ml FC/FACS: 0.5-1 ug/10^6 IHC: 5-10 ug/ml. Researchers should empirically determine the suitability of the CASP8 casp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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