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Testing Data (Fig-1: Expression analysis of Vimentin. Anti-Vimentin antibody (11-7555) was used at 1 ug/ml on A549 lysate.)

Rabbit anti-Human Vimentin Polyclonal Antibody | anti-VIM antibody

Polyclonal Antibody to Vimentin

Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human
Applications
Western Blot
Purity
Purified
Protein A Chromatography
Synonyms
Vimentin; Polyclonal Antibody; Polyclonal Antibody to Vimentin; VIM; Antibody to Vimentin; Anti-Vimentin Antibody; Vimentin Antibody; anti-VIM antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified
Protein A Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
466
Applicable Applications for anti-VIM antibody
Western Blot (WB)
Application Notes
WB: 1-2 ug/ml
Immunogen Information
A partial length recombinant Vimentin protein (amino acids 52-231) was used as the immunogen for this antibody.
Antigen
Vimentin
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Testing Data

(Fig-1: Expression analysis of Vimentin. Anti-Vimentin antibody (11-7555) was used at 1 ug/ml on A549 lysate.)

Testing Data (Fig-1: Expression analysis of Vimentin. Anti-Vimentin antibody (11-7555) was used at 1 ug/ml on A549 lysate.)
Related Product Information for anti-VIM antibody
Vimentin is a major constituent of the intermediate filament family of proteins whose expression correlates with increased metastatic disease, reduced patient survival and poor prognosis across multiple tumor types. It is ubiquitously expressed in normal mesenchymal cells and is known to maintain cellular integrity and provide resistance against stress. Vimentin is overexpressed in various epithelial cancers, including prostate cancer, gastrointestinal tumors, tumors of the central nervous system, breast cancer, malignant melanoma, and lung cancer. Vimentin shows dynamically altered expression patterns during different developmental stages and high sequence homology throughout all vertebrates, suggesting that the protein is physiologically important. Its overexpression in cancer correlates with accelerated tumor growth, invasion, and poor prognosis; however, the role of vimentin in cancer progression remains obscure. In the recent years, vimentin has gained much importance as a marker for epithelial-mesenchymal transition (EMT).
Product Categories/Family for anti-VIM antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
53,652 Da
NCBI Official Full Name
Vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
HEL113; CTRCT30
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
Protein Family
UniProt Gene Name
VIM

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Uniprot Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Research Articles on VIM

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Product Notes

The VIM vim (Catalog #AAA668840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Vimentin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1-2 ug/ml. Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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