anti-Human TLR5 Monoclonal Antibody | anti-TLR5 antibody

TLR5 Monoclonal Antibody

Cat. Num. AAA668196

• Gene Names: TLR5; SLE1; TIL3; SLEB1; MELIOS
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry
• Purity: Protein G Chromatography
• Synonyms: TLR5; Monoclonal Antibody; TLR5 Monoclonal Antibody; anti-TLR5 antibody

• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a Kappa
• Clone Number: ABM22G1
• Purity/Purification: Protein G Chromatography
• Form/Format: Purified; 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic

• Applicable Applications for anti-TLR5 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC)
• Application Notes: Immunohistochemical analysis: 10 ug/ml; FACS analysis: 0.5 ug/10^6 cells
• Immunogen: A partial length recombinant TLR5 protein was used as the immunogen for this antibody
• Preparation and Storage: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoidrepeated freeze and thaw cycles

Immunohistochemistry (IHC)

(Fig-1 : Immunohistochemical analysis of TLR5 in human skin tissue using TLR5antibody (Clone: ABM22G1) at 10 ?g/ml.)

Flow Cytometry (FC)

(Fig-2: Intracellular flow analysis of hTLR5 in Monocytes using 0.5 ?g/10^6 cells ofhTLR5 antibody (Clone: ABM22G1). Green represents isotype control; red represent santi-hTLR5 antibody. Goat anti-mouse PE conjugate was used as secondary.)

Flow Cytometry (FC)

(Fig-3:Intracellular flow analysis of hTLR5 antibody in TLR5 Transfected Cell line using0.5 ?g/10^6 cells of hTLR5 antibody (Clone: ABM22G1). Green represents isotypecontrol; red represents anti-hTLR5 antibody. Goat anti-mouse FITC conjugate wasused as secondary antibody.)

Related Product Information for anti-TLR5 antibody

Toll-like receptor 5 (TLR5) plays a fundamental role in pathogen recognition and downstream signaling to induce effectiveimmunity. It is well expressed in major old tissues including lung, skin, intestine, and spleen. TLR5 is present in synovial ofpatients with rheumatoid arthritis (RA), act as novel mediator of innate immunity-induced osteoclastogenesis and bone loss.TLR5 is a receptor for flagellin and is present on the basolateral surface of intestinal epithelial cells. It is involved in the migrationof intestinal epithelial cells through activation of the p38 MAPK pathway. TLR5-mediated signaling specifically enhanced thematuration of lung dendritic cells. TLR5 influences the progression and outcome of ovarian, sarcoma, and luminal breasttumors. Mutations in TLR5 have been associated with both resistance and susceptibility to systemic lupus erythematosus, andsusceptibility to Legionnaire disease.

Product Categories/Family for anti-TLR5 antibody

TLR

NCBI and Uniprot Product Information

• NCBI GI #: 16751843
• NCBI GeneID: 7100
• NCBI Accession #: NP_003259.2
• NCBI GenBank Nucleotide #: NM_003268.5
• UniProt Accession #: O60602; O15456; Q32MI2; Q32MI3; B1AZ05; B3Y633; B9VJ63; D1CS80; D3DTB8
• NCBI Official Full Name: toll-like receptor 5
• NCBI Official Synonym Full Names: toll-like receptor 5
• NCBI Official Symbol: TLR5
• NCBI Official Synonym Symbols: SLE1; TIL3; SLEB1; MELIOS
• NCBI Protein Information: toll-like receptor 5
• UniProt Protein Name: Toll-like receptor 5
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR5
• UniProt Synonym Gene Names: TIL3
• UniProt Entry Name: TLR5_HUMAN

NCBI Description

This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]

Uniprot Description

TLR5: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: plasma membrane; integral to membrane

Molecular Function: transmembrane receptor activity; interleukin-1 receptor binding

Biological Process: positive regulation of interleukin-8 production; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; male gonad development; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; inflammatory response; positive regulation of toll-like receptor signaling pathway; toll-like receptor 10 signaling pathway

Disease: Legionnaire Disease, Susceptibility To; Systemic Lupus Erythematosus, Susceptibility To, 1; Melioidosis, Susceptibility To