Mouse Dnmt3a Monoclonal Antibody | anti-Dnmt3a antibody

Dnmt3a Monoclonal Antibody

Cat. Num. AAA668016

• Gene Names: DNMT3A; TBRS; DNMT3A2; M.HsaIIIA
• Reactivity: Human (Predicted Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Dnmt3a; Monoclonal Antibody; Dnmt3a Monoclonal Antibody; anti-Dnmt3a antibody

• Host: Mouse
• Reactivity: Human (Predicted Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, Kappa
• Clone Number: ABM1F35
• Form/Format: Purified (Liquid)
• Concentration: 0.5 mg/ml, 200 µl (varies by lot)
• Sequence Length: 912

• Applicable Applications for anti-Dnmt3a antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Antigen: Dnmt3a
• Immunogen: Full lengtgh recombinant DNMT3a protein was used as the immunogen for this antibody
• Preparation and Storage: Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.

Western Blot (WB)

(Expression analysis of DNMT3a. Anti-DNMT3a antibody (Clone: ABM1F35) was used at 2 µg/ml Hek 293 Lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Dnmt3a in normal human liver tissue using Dnmt3a antibody (Clone: ABM1F35) at 5 ug/ml.)

Product Categories/Family for anti-Dnmt3a antibody

DNA

NCBI and Uniprot Product Information

• NCBI GI #: 12751473
• NCBI GeneID: 1788
• NCBI Accession #: NP_072046.2
• NCBI GenBank Nucleotide #: NM_022552.4
• UniProt Accession #: Q9Y6K1; Q86TE8; Q86XF5; Q8IZV0; Q8WXU9; E9PEB8
• Molecular Weight: 16,866 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3A isoform a
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3 alpha
• NCBI Official Symbol: DNMT3A
• NCBI Official Synonym Symbols: TBRS; DNMT3A2; M.HsaIIIA
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3A
• Protein Family: DNA
• UniProt Gene Name: DNMT3A
• UniProt Synonym Gene Names: Dnmt3a; DNA MTase HsaIIIA; M.HsaIIIA
• UniProt Entry Name: DNM3A_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase, DNA; Methyltransferase; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.37

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: nucleoplasm; XY body; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; identical protein binding; protein binding; DNA binding; unmethylated CpG binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; metal ion binding; chromatin binding; DNA-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; genetic imprinting; DNA methylation during gametogenesis; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; cytosine methylation within a CG sequence; methylation-dependent chromatin silencing; negative regulation of gene expression, epigenetic; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; gene expression; DNA methylation during embryonic development; DNA methylation on cytosine

Disease: Tatton-brown-rahman Syndrome