Western Blot (WB)
(Western blot analysis of Acetyl- and Phospho-Histone H3 (Lys9/Ser10) in control and serum, calyculin A and TSA treated NIH/3T3 cell extracts of Acetyl- and Phospho-Histone H3 (Lys9/Ser10) alone (left) and the same antibody preincubated with Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Blocking Peptide (right).)
Histone H3, acetylated Peptide | TTHERM_00189180 peptide
Histone H3, acetylated (Lys9), phosphorylated (Ser10) Blocking Peptide
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
PurifiedPurified
Synonyms
Histone H3; acetylated; acetylated (Lys9); phosphorylated (Ser10) Blocking Peptide; TTHERM_00189180 peptide
Specificity
Note: Adherence to the attached protocol is extremely important.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a lyophilized powder.
Applicable Applications for TTHERM_00189180 peptide
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of Acetyl- and Phospho-Histone H3 (Lys9/Ser10) in control and serum, calyculin A and TSA treated NIH/3T3 cell extracts of Acetyl- and Phospho-Histone H3 (Lys9/Ser10) alone (left) and the same antibody preincubated with Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Blocking Peptide (right).)
Western Blot (WB)
(Western blot analysis of Acetyl- and Phospho-Histone H3 (Lys9/Ser10) in control and serum, calyculin A and TSA treated NIH/3T3 cell extracts of Acetyl- and Phospho-Histone H3 (Lys9/Ser10) alone (left) and the same antibody preincubated with Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Blocking Peptide (right).)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded human breast carcinoma, using Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody #9711 preincubated with irrelevant control peptide (left) and Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Blocking Peptide (right).)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded human breast carcinoma, using Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody #9711 preincubated with irrelevant control peptide (left) and Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Blocking Peptide (right).)
Related Product Information for TTHERM_00189180 peptide
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different post-translational modifications including acetylation, phosphorylation and methylation (2-4). These modifications occur in
Produced by immunizing rabbits with a synthetic acetylated and phosphorylated peptide, corresponding to residues surrounding Lys9 and Ser10 of human histone H3.
Produced by immunizing rabbits with a synthetic acetylated and phosphorylated peptide, corresponding to residues surrounding Lys9 and Ser10 of human histone H3.
Product Categories/Family for TTHERM_00189180 peptide
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TTHERM_00189180 (Catalog #AAA659758) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Histone H3, acetylated can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Researchers should empirically determine the suitability of the TTHERM_00189180 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, acetylated, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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