Neprilysin-Like Protease-a Peptide
Neprilysin-Like Protease-a, Mouse (NEPL-a), Control Peptide
Applications
ELISA
Purity
Highly PurifiedHighly purified
Synonyms
Neprilysin-Like Protease-a; Mouse (NEPL-a); Control Peptide; Neprilysin-Like Protease-a peptide
Host
Mouse
Specificity
100% conserved in mouse NEPL-a, b and g. No significant sequence homology seen with other proteins.
Purity/Purification
Highly Purified
Highly purified
Highly purified
Form/Format
Supplied as a liquid in PBS, pH 7.5.
Applicable Applications for Neprilysin-Like Protease-a peptide
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA and Antibody Blocking. Because of its low MW (<3kD), not suitable for Western Blot., , Recommended Dilutions:, ELISA: 50-100ng of control peptide/well, Antibody Blocking: 5-10ug per 1ug of N2030-32 (purified IgG) or 1ul of N2030-30 (antiserum). Optimal dilutions to be determined by the researcher.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Neprilysin-Like Protease-a peptide
A 17-aa peptide sequence within the cytoplasmic N-terminus of mouse neprilysin-like protease-a.
The amyloid beta-peptide (Ab) of 39 to 43aa (Ab40, Ab42, Ab43) is constitutively produced in brain upon proteolysis of the b-amyloid precursor protein (APP). In the young and healthy humans, Ab is rapidly catabolized before it can be deposited in the brain. However, upon aging or the onset of familial Alzheimer's Disease, alterations in either synthesis or degradation/clearance of Ab may contribute to amyloid depositions in the brain. Ab is susceptible to a number of in vivo and in vitro proteases like cathepsin-D and M-13 metalloproteases. The M-13 family includes several members of zinc-dependent proteases like damage-induced neuronal endopeptidase (DINE), product of phosphate regulating gene with homologies to endopeptidases on the X chromosome (PHEX), neutral endopeptidase 24.11 or neprilysin (NEP) and neprilysin-like proteases (NEPLs). NEPLs (alpha, -beta, gamma) arise from the alternative splicing of a single NELPS gene and show ~54% sequence homology. M13 members are generally type II transmembrane proteins consisting of a single polypeptide chain with Zinc binding HEXXH motif, a short cytoplasmic tail, a transmembrane segment and an extra-cytoplasmic domain containing the enzyme active site.
NEPL-a (variously called SEPD/splice 1) is a type II trans-membrane metallopeptidase (~100kD non-glycosylated and 120kD glycosylated) containing a highly glycosylated polypeptide chain of 742aa with a HEXXH zinc-binding consensus, cytoplasmic tail and a large extracellular catalytic domain. NPL-a lacks a 23aa sequence present in N-terminal of NEPL-b. In contrast to NEP and NEPL-b which exist in membrane-bound as well as soluble forms, NEPL-a is expressed as membrane-bound form only. In comparison to NEP, the NEPL-a has lower affinity and/or smaller Vmax for Ab and cannot proteolyze cell secreted Ab40 or Ab42 in vivo. Unlike NEP, NEPL-a is not a major in vivo peptidase for degrading endogenous Ab however, it might degrade Ab in Alzheimer patients where Ab is accumulated in excess and an Ab-degradative pathway, alternative to NEP, exists.
The amyloid beta-peptide (Ab) of 39 to 43aa (Ab40, Ab42, Ab43) is constitutively produced in brain upon proteolysis of the b-amyloid precursor protein (APP). In the young and healthy humans, Ab is rapidly catabolized before it can be deposited in the brain. However, upon aging or the onset of familial Alzheimer's Disease, alterations in either synthesis or degradation/clearance of Ab may contribute to amyloid depositions in the brain. Ab is susceptible to a number of in vivo and in vitro proteases like cathepsin-D and M-13 metalloproteases. The M-13 family includes several members of zinc-dependent proteases like damage-induced neuronal endopeptidase (DINE), product of phosphate regulating gene with homologies to endopeptidases on the X chromosome (PHEX), neutral endopeptidase 24.11 or neprilysin (NEP) and neprilysin-like proteases (NEPLs). NEPLs (alpha, -beta, gamma) arise from the alternative splicing of a single NELPS gene and show ~54% sequence homology. M13 members are generally type II transmembrane proteins consisting of a single polypeptide chain with Zinc binding HEXXH motif, a short cytoplasmic tail, a transmembrane segment and an extra-cytoplasmic domain containing the enzyme active site.
NEPL-a (variously called SEPD/splice 1) is a type II trans-membrane metallopeptidase (~100kD non-glycosylated and 120kD glycosylated) containing a highly glycosylated polypeptide chain of 742aa with a HEXXH zinc-binding consensus, cytoplasmic tail and a large extracellular catalytic domain. NPL-a lacks a 23aa sequence present in N-terminal of NEPL-b. In contrast to NEP and NEPL-b which exist in membrane-bound as well as soluble forms, NEPL-a is expressed as membrane-bound form only. In comparison to NEP, the NEPL-a has lower affinity and/or smaller Vmax for Ab and cannot proteolyze cell secreted Ab40 or Ab42 in vivo. Unlike NEP, NEPL-a is not a major in vivo peptidase for degrading endogenous Ab however, it might degrade Ab in Alzheimer patients where Ab is accumulated in excess and an Ab-degradative pathway, alternative to NEP, exists.
Product Categories/Family for Neprilysin-Like Protease-a peptide
Similar Products
Product Notes
The Neprilysin-Like Protease-a (Catalog #AAA658638) is a Peptide produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Neprilysin-Like Protease-a can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA and Antibody Blocking. Because of its low MW (<3kD), not suitable for Western Blot.,, Recommended Dilutions:, ELISA: 50-100ng of control peptide/well, Antibody Blocking: 5-10ug per 1ug of N2030-32 (purified IgG) or 1ul of N2030-30 (antiserum). Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Neprilysin-Like Protease-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neprilysin-Like Protease-a, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
