HNE Native Protein
HNE (4-Hydroxy-2-nonenal), Western Blot Control (BSA)
Applications
Western Blot
Purity
PurifiedPurified
Synonyms
HNE; HNE (4-Hydroxy-2-nonenal); Western Blot Control (BSA); HNE native protein
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied in 100ul of SDS-PAGE sample buffer (reduced).
Applicable Applications for HNE native protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot as positive control. Not suitable for use in ELISA.
Dilution: Western Blot: 10 ul/lane. Loading volume may be adjusted depending upon the intensity of the band under actual experimental condition. BSA-HNE conjugate is ~65-70kD. Other aggregated bands may be visible as well. BSA-HNE conjugate should only be used as a positive control to optimize the blotting conditions. It is not a representative of the protein bands that might be labeled with the anti-HNE in a given samples. Typically, complex proteins samples, such as liver or brain, will show many bands that are modified with HNE. There is no specific pattern that must be obtained with the antibodies.
Dilution: Western Blot: 10 ul/lane. Loading volume may be adjusted depending upon the intensity of the band under actual experimental condition. BSA-HNE conjugate is ~65-70kD. Other aggregated bands may be visible as well. BSA-HNE conjugate should only be used as a positive control to optimize the blotting conditions. It is not a representative of the protein bands that might be labeled with the anti-HNE in a given samples. Typically, complex proteins samples, such as liver or brain, will show many bands that are modified with HNE. There is no specific pattern that must be obtained with the antibodies.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for HNE native protein
HNE is a major product of endogenous lipid peroxidation. The w-6-family (linoleic and arachidonic acids) of polyunsaturated fatty acids may produce HNE as a result of free radical attack. HNE is a highly reactive compound and it can react with several functional groups on biological material, particularly sulfydryl groups to form thioester adduct and then hemiacetals. HNE may also react with histidine and lysine residues of proteins to form stable Michael addition-type of adducts. HNE-modified proteins may display an altered biological functions. Antibodies to HNE will help to visualize the HNE-adducts.
Product Categories/Family for HNE native protein
Similar Products
Product Notes
The HNE (Catalog #AAA656952) is a Native Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HNE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot as positive control. Not suitable for use in ELISA. Dilution: Western Blot: 10 ul/lane. Loading volume may be adjusted depending upon the intensity of the band under actual experimental condition. BSA-HNE conjugate is ~65-70kD. Other aggregated bands may be visible as well. BSA-HNE conjugate should only be used as a positive control to optimize the blotting conditions. It is not a representative of the protein bands that might be labeled with the anti-HNE in a given samples. Typically, complex proteins samples, such as liver or brain, will show many bands that are modified with HNE. There is no specific pattern that must be obtained with the antibodies. Researchers should empirically determine the suitability of the HNE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNE, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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