Maackia amurensis Lectin II Lectin
Maackia amurensis Lectin II (MAL II) (Biotin)
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Molecular Biology Grade
Synonyms
Maackia amurensis Lectin II; Maackia amurensis Lectin II (MAL II) (Biotin); Maackia amurensis Lectin II lectin
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide. Labeled with Biotin.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for Maackia amurensis Lectin II lectin
ELISA, Western Blot, Immunohistochemistry
Application Notes
Optimal dilutions to be determined by the researcher.
Inhibitor
Human glycophorin
Lectin Biotinylated
98%
Sugar Specificity
Sialic Acid
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Maackia amurensis Lectin II lectin
Maackia amurensis lectin II (MAH) is the hemagglutinin isolectin from Maakia seeds. It is a glycoprotein consisting of four subunits. The molecular weight of the lectin is about 140,000 resolvable by SDS gel electrophoresis into two bands of 34,000 and 36,000D. The isoelectric point of this lectin is about pH 4.7 but demonstrates some charge heterogeneity. Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike SNA which seems to prefer structures with (a-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (a-2,3) linkage. While fetuin is a poor inhibitor, glycophorin is a very potent inhibitor of MAL II binding. Tissue staining patterns are also very different among MAL I, SNA and MAL II. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.
Product Categories/Family for Maackia amurensis Lectin II lectin
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Product Notes
The Maackia amurensis Lectin II (Catalog #AAA656000) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Maackia amurensis Lectin II can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Maackia amurensis Lectin II for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Maackia amurensis Lectin II, Lectin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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