Chymotrypsin Pancreas, Inhibitor | CTRC, inhibitor

Chymotrypsin, Human Pancreas

Cat. Num. AAA655348

• Gene Names: CTRC; CLCR; ELA4
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: Chymotrypsin Pancreas; Chymotrypsin; Human Pancreas; CTRC; inhibitor

• Host: Human pancreatic tissue
• Specificity: In addition to bonds involving aromatic amino acids, chymotrypsin catalyzes at a high rate the hydrolysis of bonds of leucyl, methionyl, asparaginyl, and glutamyl residues. A recent study has been made by Berezin and Martinek (1970) and Baumann et al. (1970).
• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a salt free, lyophilized powder

• Application Notes: Suitable for antigenic applications in immunological protocols. Shows no trypsin activity when assayed against N-benzyl-L-arginine-p-nitroanilide.
• CAS Number: 9004-07-3
• Extinction Coefficient: 20.4
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CTRC, inhibitor

Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues. Chymotrypsinogen A is extracted from pancreatic tissue as a zymogen. Pancreatic extract contains approximately equal amounts of two forms of the zymogen, chymotrypsin A and chymotrypsin B. Chymotrypsin A may be activated to alpha, pi, beta, or gamma chymotrypsin. The enzyme is inhibited by heavy metals, the natural trypsin inhibitors to various degrees, an inhibitor from potato, and organophosphorous compounds.

Product Categories/Family for CTRC, inhibitor

Molecular Biology; MB-Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 62526043
• NCBI GeneID: 11330
• NCBI Accession #: NP_009203.2
• NCBI GenBank Nucleotide #: NM_007272.2
• UniProt Accession #: Q99895; O00765; Q9NUH5; A8K082
• Molecular Weight: 29,484 Da
• NCBI Official Full Name: chymotrypsin-C preproprotein
• NCBI Official Synonym Full Names: chymotrypsin C (caldecrin)
• NCBI Official Symbol: CTRC
• NCBI Official Synonym Symbols: CLCR; ELA4
• NCBI Protein Information: chymotrypsin-C; caldecrin; elastase 4; elastase IV; serum calcium decreasing factor
• UniProt Protein Name: Chymotrypsin-C
• Protein Family: Chymotrypsin
• UniProt Gene Name: CTRC
• UniProt Synonym Gene Names: CLCR
• UniProt Entry Name: CTRC_HUMAN

NCBI Description

This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Ref.8

Catalytic activity: Preferential cleavage: Leu-|-Xaa, Tyr-|-Xaa, Phe-|-Xaa, Met-|-Xaa, Trp-|-Xaa, Gln-|-Xaa, Asn-|-Xaa.

Tissue specificity: Pancreas.

Involvement in disease: Genetic variations in CTRC can predispose to chronic pancreatitis by diminishing its protective trypsin-degrading activity.

Sequence similarities: Belongs to the peptidase S1 family. Elastase subfamily.Contains 1 peptidase S1 domain.