Influenza B Antigen, Strain Hong Kong 5/72 Native Protein
Influenza B Antigen, Strain Hong Kong 5/72
Applications
ELISA
Purity
Clarified and concentrated cell culture supernatant
Synonyms
Influenza B Antigen; Strain Hong Kong 5/72; Strain Hong Kong 5/72 native protein
Host
MDCK cellsStrain:B/Hong Kong 5/72
Purity/Purification
Clarified and concentrated cell culture supernatant
Form/Format
Purified Liquid
Concentration
1.2 mg/mL (Dye Binding Assay)
HA Titer: 20480 HA Units/mL (Hemmagglutination Endpoint Assay) (varies by lot)
HA Titer: 20480 HA Units/mL (Hemmagglutination Endpoint Assay) (varies by lot)
Applicable Applications for Influenza B Antigen, Strain Hong Kong 5/72 native protein
ELISA (EL/EIA)
Application Notes
This preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format. May be used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded
Buffer: EMEM
NOTE: Freezing a buffered protein solution such as EMEM may selectively crystallize one buffering species, causing pH changes. EMEM (pH 7) is red in color (due to phenol red) at room temperature. Disodium Phosphate component crystallizes more readily than Monosodium Phosphate on freezing because the solubility of the disodium form is considerably lower than that of the monosodium form. Because of this, a solution that is buffered by sodium phosphate buffer at pH 7 at room temperature (and is red in color) may experience a pH drop during freezing and become orange or yellow. On thawing the material will be red in color because disodium salt will be re-solubilized and pH will be 7.
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded
Buffer: EMEM
NOTE: Freezing a buffered protein solution such as EMEM may selectively crystallize one buffering species, causing pH changes. EMEM (pH 7) is red in color (due to phenol red) at room temperature. Disodium Phosphate component crystallizes more readily than Monosodium Phosphate on freezing because the solubility of the disodium form is considerably lower than that of the monosodium form. Because of this, a solution that is buffered by sodium phosphate buffer at pH 7 at room temperature (and is red in color) may experience a pH drop during freezing and become orange or yellow. On thawing the material will be red in color because disodium salt will be re-solubilized and pH will be 7.
Source
MDCK Cells
Inactivation
Gamma radiation inactivation. Inactivated sample is inoculated onto MDCK cells alongside positive and negative controls and serially passaged after three days incubation. Result: No growth detected.
Preparation and Storage
Store at -70°C to -100°C. Avoid multiple freeze/thaw cycles.
Related Product Information for Influenza B Antigen, Strain Hong Kong 5/72 native protein
Influenza is an acute infection of the respiratory tract caused by the Influenza virus, of which there are several different types. Influenza B is only known to infect humans and seals. Influenza infection in humans causes chills, fever, pharyngitis, muscle pain, headaches, coughing and weakness. The virus is transmitted through the air by coughs or sneezes or through saliva, nasal secretions, faeces and blood. Infection may occur through direct contact with infected bodily fluid or contaminated surfaces. Influenza B viruses mutate slower than Influenza A, but fast enough that lasting immunity is not possible. The slower mutation rate and limited host range makes Influenza B less like to cause pandemics than Influenza A.
Product Categories/Family for Influenza B Antigen, Strain Hong Kong 5/72 native protein
Similar Products
Product Notes
The Influenza B Antigen, Strain Hong Kong 5/72 (Catalog #AAA655300) is a Native Protein produced from MDCK cellsStrain:B/Hong Kong 5/72 and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Influenza B Antigen, Strain Hong Kong 5/72 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). This preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format. May be used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded Buffer: EMEM NOTE: Freezing a buffered protein solution such as EMEM may selectively crystallize one buffering species, causing pH changes. EMEM (pH 7) is red in color (due to phenol red) at room temperature. Disodium Phosphate component crystallizes more readily than Monosodium Phosphate on freezing because the solubility of the disodium form is considerably lower than that of the monosodium form. Because of this, a solution that is buffered by sodium phosphate buffer at pH 7 at room temperature (and is red in color) may experience a pH drop during freezing and become orange or yellow. On thawing the material will be red in color because disodium salt will be re-solubilized and pH will be 7. Researchers should empirically determine the suitability of the Influenza B Antigen, Strain Hong Kong 5/72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Influenza B Antigen, Strain Hong Kong 5/72, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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