RIPA Lysis Buffer
RIPA Lysis Buffer
Applications
Western Blot, Immunoprecipitation
Purity
Molecular Biology Grade
Synonyms
RIPA Lysis Buffer; RIPA Lysis Buffer buffer
Purity/Purification
Molecular Biology Grade
Applicable Applications for RIPA Lysis Buffer buffer
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Dilution: Immediately prior to use, add the protease and phosphatase inhibitors for the following final concentrations: 1mM PMSF, 1mM sodium orthovanadate, 1mM sodium fluoride, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin
pH
7.5 ± 0.5
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 6 months. Store in the dark.
Related Product Information for RIPA Lysis Buffer buffer
RIPA lysis buffer is used to lyse cultured cells for immunoprecipitation and immunoblots. Similar in function to single detergent buffers that include NP-40, sodium chloride and Tris, RIPA is a good first choice for new antigens.
Product Categories/Family for RIPA Lysis Buffer buffer
Similar Products
Product Notes
The RIPA Lysis Buffer (Catalog #AAA651650) is a Buffer and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RIPA Lysis Buffer can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Dilution: Immediately prior to use, add the protease and phosphatase inhibitors for the following final concentrations: 1mM PMSF, 1mM sodium orthovanadate, 1mM sodium fluoride, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin. Researchers should empirically determine the suitability of the RIPA Lysis Buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIPA Lysis Buffer, Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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