Interleukin 19 Active Protein | IL19 active protein
Interleukin 19, Recombinant, Human (IL-19, Melanoma Differentiation Associated like Protein)
Gene Names
IL19; MDA1; NG.1; ZMDA1; IL-10C
Purity
Highly Purified98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Synonyms
Interleukin 19; Recombinant; Human (IL-19; Melanoma Differentiation Associated like Protein); IL19 active protein
Host
E Coli
Purity/Purification
Highly Purified
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form/Format
Supplied as a lyophilized powder in 10mM sodium citrate, pH 3.0.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Cys.
Biological Activity
Recombinant Human Interleukin-19 is fully biologically active when compared to standard. The activity is determined by it's ability to activate STAT following receptor-ligand interaction.
Dimers and Aggregates
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of human IL-19.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard.
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-19 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
-20 degree C
Stability: Lyophilized Interleukin-19 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human IL-19 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Stability: Lyophilized Interleukin-19 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human IL-19 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Related Product Information for IL19 active protein
Interleukin-19 (IL-19) is a novel cytokine that was initially identified during a sequence data base search aimed at finding potential Interleukin-10 homologs. Interleukin-19 shares 21% amino acid identity with IL-10. The exon/intron structure of IL-19 is similar to that of the human IL-10 gene, comprising five exons and four introns within the coding region of the IL-19 cDNA. IL-19 shares a receptor complex with IL-20, indicating that the biological activities of these two cytokines overlap and that both may play an important role in regulating development and proper functioning of the skin. Unlike IL-10, which forms an intercalated dimer, the molecule of IL-19 is a monomer made of seven amphipathic helices, A-G, creating a unique helical bundle.
Recombinant Human IL-19 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids and having a molecular mass of 17913 Dalton. Recombinant Human Interleukin-19 is purified by proprietary chromatographic techniques.
Recombinant Human IL-19 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids and having a molecular mass of 17913 Dalton. Recombinant Human Interleukin-19 is purified by proprietary chromatographic techniques.
Product Categories/Family for IL19 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17.91kD
NCBI Official Full Name
interleukin 19
NCBI Official Synonym Full Names
interleukin 19
NCBI Official Symbol
IL19
NCBI Official Synonym Symbols
MDA1; NG.1; ZMDA1; IL-10C
NCBI Protein Information
interleukin-19; OTTHUMP00000034568; OTTHUMP00000216804; melanoma differentiation associated protein-like protein; melanoma differentiation-associated protein-like protein
UniProt Protein Name
Interleukin 19
Protein Family
UniProt Gene Name
IL19
UniProt Entry Name
Q5VUT3_HUMAN
NCBI Description
The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq]
Uniprot Description
IL19: May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis. Belongs to the IL-10 family.
Protein type: Apoptosis; Secreted; Secreted, signal peptide; Cytokine
Chromosomal Location of Human Ortholog: 1q32.2
Cellular Component: extracellular space; extracellular region
Molecular Function: cytokine activity
Biological Process: apoptosis; positive regulation of JAK-STAT cascade; immune response; inflammatory response; signal transduction; interleukin-6 biosynthetic process
Research Articles on IL19
1. IL-19 is mitogenic and chemotactic for endothelial cells and can induce their angiogenic potential.
Similar Products
Product Notes
The IL19 il19 (Catalog #AAA650867) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Ar g-Arg-Cys. It is sometimes possible for the material contained within the vial of "Interleukin 19, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
