Interleukin 4, Rat Active Protein | IL4 active protein
Interleukin 4, Recombinant, Rat (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitra
Purity
Highly Purified98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Synonyms
Interleukin 4; Rat; Recombinant; Rat (IL-4; B Cell Stimulatory Factor 1; BSF1; Binetrakin; HCGF; Hodgkin's Cell Growth Factor; IA Inducing Factor; Lymphocyte Stimulatory Factor 1; Macrophage Fusion Factor; Mast Cell Growth Factor 2; MCGF2; MFF; MGC79402; Pitra; IL4 active protein
Host
E Coli
Purity/Purification
Highly Purified
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form/Format
Supplied as a lyophilized powder without additives.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Cys Asn.
Biological Activity
Recombinant Rat IL-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent prolifiration of Con A activated rat spleen cells is 1.5ng/ml, corresponding to a Specific Activity of 6.6 x 10e5 IU/mg.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of Interleukin-4
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
Solubility
It is recommended to reconstitute the lyophilized IL-4 in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
-20 degree C
Stability: Recombinant Rat IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution, Interleukin-4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Stability: Recombinant Rat IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution, Interleukin-4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Related Product Information for IL4 active protein
Interleukin-4 (IL4) has a compact, globular fold (similar to other cytokines), stabilized by 3 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. The helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet.
Interleukin (IL)-4, produced primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumor cells. IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses.
Recombinant Rat IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 14105D.
Interleukin (IL)-4, produced primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumor cells. IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses.
Recombinant Rat IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 14105D.
Product Categories/Family for IL4 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.11kD
NCBI Official Full Name
interleukin-4
NCBI Official Symbol
IL4
NCBI Protein Information
interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
UniProt Protein Name
Interleukin-4
Protein Family
UniProt Gene Name
IL4
UniProt Entry Name
IL4_PIG
Uniprot Description
Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-4/IL-13 family.
