Interleukin 13 Active Protein | IL13 active protein

Interleukin 13, Recombinant, Mouse (IL-13)

Cat. Num. AAA650801

• Gene Names: IL13; ALRH; BHR1; P600; IL-13; MGC116786; MGC116788; MGC116789
• Purity: Highly Purified; 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: Interleukin 13; Recombinant; Mouse (IL-13); IL13 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Supplied as a lyophilized powder without additives.
• Sequence: The sequence of the first five N-terminal amino acids was determined and found to be Met-Pro-Val-Pro-Arg.

• Biological Activity: Recombinant IL-13 is fully biologically active when compared to standard. The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be < 4ng/ml, corresponding to a specific activity of >2.5 x 105.
• Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of recombinant mouse IL-13.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a calibrated solution of Interleukin-13 as a Reference Standard.
• Solubility: It is recommended to reconstitute the lyophilized Recombinant IL-13 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: -20 degree C; Stability: Lyophilized IL-13 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-13 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Related Product Information for IL13 active protein

Interleukin-13 (IL-13) is a multifunctional cytokine whose net principle action is to diminish inflammatory responses. Interleukin (IL)-13 is an important cytokine secreted from type 2 helper T lymphocytes. It is essential for modulating IgE synthesis by human B cells. Previous studies showed that polymorphisms in the IL-13 gene were associated with serum total IgE or allergic asthma. Interleukin (IL)-13 is a pleiotropic cytokine, which shares many biological functions with IL-4. The receptor subunits of IL-13 consist of IL-4Ralpha, IL-13Ralpha1 and IL-13Ralpha2.

Recombinant interleukin-13 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12127 Dalton. Mouse IL-13 is purified by proprietary chromatographic techniques.

Product Categories/Family for IL13 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 26787978
• NCBI GeneID: 3596
• NCBI Accession #: NP_002179.2
• NCBI GenBank Nucleotide #: NM_002188.2
• UniProt Accession #: P35225; O43644; Q4VB52; Q9UDC7; Q4VB50; Q4VB51; Q4VB53
• Molecular Weight: 12.13kD
• NCBI Official Full Name: interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: IL13
• NCBI Official Synonym Symbols: ALRH; BHR1; P600; IL-13; MGC116786; MGC116788; MGC116789
• NCBI Protein Information: interleukin-13; allergic rhinitis; OTTHUMP00000066048; Bronchial hyperresponsiveness-1 (bronchial asthma)
• UniProt Protein Name: Interleukin-13
• Protein Family: Interleukin
• UniProt Gene Name: IL13
• UniProt Synonym Gene Names: NC30
• UniProt Entry Name: IL13_HUMAN

NCBI Description

This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq]

Uniprot Description

Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.

Subunit structure: Interacts with IL13RA2.

Subcellular location: Secreted.

Involvement in disease: Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH) [

MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.

Sequence similarities: Belongs to the IL-4/IL-13 family.

Caution: It is uncertain whether Met-1 or Met-15 is the initiator.

Sequence caution: The sequence AAA36107.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA83738.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAB01681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAC03535.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAK53823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAA48824.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.