Bone Morphogenetic Protein 7, Recombinant, Human, Western Blot Positive Control Recombinant Protein | BMP7 recombinant protein

Bone Morphogenetic Protein 7, Recombinant, Human, Western Blot Positive Control (BMP7)

Cat. Num. AAA650711

• Gene Names: BMP7; OP-1
• Applications: Western Blot
• Purity: Purified
• Synonyms: Bone Morphogenetic Protein 7; Recombinant; Human; Western Blot Positive Control; Western Blot Positive Control (BMP7); BMP7; BMP7 recombinant protein

• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in SDS-PAGE sample buffer.

• Applicable Applications for BMP7 recombinant protein: Western Blot
• Application Notes: Recommended Dilutions: Western Blot: Load 10ul/lane of human BMP-6 for good visibility with antibodies.
• Source Information: Recombinant Human BMP-7P-2 signal peptide and propeptides (aa1-282 of human BMP-2) fused to the human BMP-7 mature chain (aa293-431 of human BMP-7) was expressed in Chinese hamster ovary cell line. The mature recombinant human BMP-7, generated by the proteolytic removal of the signal peptide and propeptide, is a disulfide-linked homodimeric protein consisting of two 139aa residue subunits with a MW of ~15.7kD. Due to glycosylation the rhBMP-5 migrates as an ~30-40kD protein under non-reducing conditions and as 18-20kD protein under reducing conditions in SDS-PAGE.
• Product Note: SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.; Shipping Temperature: Blue Ice

Related Product Information for BMP7 recombinant protein

Bone Morphogenetic Proteins belong to the TGF-beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type I and II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.

BMP7 or OP1, a 431-amino acid polypeptide that includes a secretory signal sequence, (Chr 20), is expressed in kidney, bladder and brain. Induces cartilage and bone formation may be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. It plays a role in calcium regulation and bone homeostasis.

Product Categories/Family for BMP7 recombinant protein

Growth Factors; Cytokines; Growth Factors-BMP

NCBI and Uniprot Product Information

• NCBI GI #: 12003264
• NCBI GeneID: 655
• UniProt Accession #: Q9H2W7; P18075; Q9NQQ8
• Molecular Weight: 15,599 Da
• NCBI Official Full Name: bone morphogenetic protein 7
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: BMP7
• NCBI Official Synonym Symbols: OP-1
• NCBI Protein Information: bone morphogenetic protein 7; BMP-7; OTTHUMP00000031357; OTTHUMP00000166092; OTTHUMP00000166093; OTTHUMP00000166094; osteogenic protein 1
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP7
• UniProt Entry Name: Q9H2W7_HUMAN