Granulocyte Macrophage Colony Stimulating Factor Active Protein | CSF2 active protein

Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

Cat. Num. AAA650678

• Gene Names: CSF2; GMCSF; MGC131935; MGC138897
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ ug (1EU/ug).
• Synonyms: Granulocyte Macrophage Colony Stimulating Factor; Recombinant; Human (GM-CSF; Burst Promoting Activity; CMCSF; Colony Stimulating Factor 2; CSF Alpha; Eosinophil Colony Stimulating Factor; Molgramostin; Pl; CSF2 active protein

• Host: Human, recombinant, E Coli
• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ ug (1EU/ug).
• Form/Format: Supplied as a sterile-filtered, lyophilized powder from 10mM sodium citrate, pH 3.5. No preservative added. Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. This solution can be diluted into other buffered solutions or stored at -20 degree C

• Biological Activity: GM-CSF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg.
• Country of Origin: USA
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Related Product Information for CSF2 active protein

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages. It promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

Recombinant human GM-CSF is a 14.6kD globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

Product Categories/Family for CSF2 active protein

Growth Factors; Cytokines; Growth Factors-GM-CSF

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_000749.2
• NCBI GenBank Nucleotide #: NM_000758.2
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6; Q647J8
• Molecular Weight: 14.6kD
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF; MGC131935; MGC138897
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; OTTHUMP00000065962; granulocyte-macrophage colony stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq]

Uniprot Description

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.