Rabbit anti-Human sTNFR2 Polyclonal Antibody

Rabbit Anti-sTNFR2

Cat. Num. AAA6506769

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Neutralization Assay, Western Blot
• Purity: Affinity Purified. Purified by immunoaffinity chromatography.
• Synonyms: sTNFR2; Polyclonal Antibody; Rabbit Anti-sTNFR2; Tumor Necrosis Factor Receptor Superfamily Member 1B; Tumor Necrosis Factor Receptor 2; TNF-R2; Tumor Necrosis Factor Receptor Type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha Receptor; CD120b; Etanercept; TNFRSF1B; TNFBR; anti-sTNFR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified. Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.

• Applicable Applications for anti-sTNFR2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Neutralization, Western Blot (WB)
• Application Notes: ELISA (Sandwich): 0.5-2ug/ml (100ul/well). Using in conjunction with MBS624679, as a detection antibody, the detection limit of recombinant human sTNF Receptor II is ~0.2-0.4ng/well.; ; Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sTNF-receptor II is 1.5-3ng/lane, under either reducing or non-reducing conditions.; ; Immunohistochemistry: 3ug/ml incubated overnight at 4 degree C followed by a fluorescent labeled secondary antibody. Stained PC-3 and CaCo-2 cells.; ; Neutralization: 15-20ug/ml yields one-half maximal inhibition of the biological activity of Human sTNF Receptor-II (0.5ug/ml).; ; Optimal dilutions should be determined by the researcher.
• Immunogen: Recombinant protein corresponding to human sTNF Receptor Type II, expressed in E.coli
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-sTNFR2 antibody

TNF RII (p75, CD120b) is a 75kD transmembrane glycoprotein originally isolated from a human lung fibroblast library. Among the multitude of cells known to express TNFRII are monocytes, endothelial cells, Langerhans cells, and macrophages. Mouse to human amino acid sequence identity in the TNFRII cytoplasmic domain is 73%, while aa sequence identity in the extracellular region falls to 58%. This drop in extracellular identity is reflected in the observation that human TNF-alpha is not active in the mouse system. TNF RII to TNF RI, amino acid sequence identity is only about 20% in the extracellular region and 5% in the cytoplasmic domain. TNF RII consists of a 240aa residue extracellular region, a 27aa residue transmembrane segment and a 173aa residue cytoplasmic domain. TNF R1 and TNF R2 are members of a family of structurally related membrane receptors that includes lymphotoxin receptor, Fas, WSL1, DR4, CD40, CD30, CD27, 4-1BB, OX40, and p75 nerve growth factor receptor. Members of the TNFR family can interact through their cytoplasmic domains with a range of intracellular signalling proteins, most of which fall into two distinct groups. The first is the death domain containing proteins, including TRADD, FADD/MORT1, and RIP, which associate directly with receptors also containing death domains, such as TNF R1 and Fas. The second is the TRAF proteins. TRAF1 and TRAF2 were originally identified by their association with the cytoplasmic domain of TNFR2. TRAF proteins appear to function as adaptor proteins. TRAF2 directly binds at least eight intracellular molecules, including TRAF1, cIAP1, cIAP2, I-TRAF/TANK, A20, TRIP, RIP, and NIK. The best characterized TRAFmediated signal transduction pathway is the activation of NF-B transcription factors. TRAF2 mediates NF-B activation via the recruitment of the serine/threonine kinase NIK, which can in turn activate CHUK, an IBspecific kinase that triggers IB degradation. In addition to recruiting mediators of NF-B activation, TRAF2 can bind at least three other molecules (I-TRAF/TANK, A20, TRIP) that inhibit its ability to activate NF-B.