IGFBP-7, Recombinant, Mouse, Western Blot Positive Control Recombinant Protein | IGFBP7 recombinant protein

IGFBP-7, Recombinant, Mouse, Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 7, IBP-7, IGF-binding Protein 7, IBP7, GFBP-rP1, MAC25 Protein, PGI2-stimulating Factor, Prostacyclin-stimulating Factor, Tumor-derived Adhesion Factor,

Cat. Num. AAA650530

• Gene Names: IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; IGFBP-7v; IGFBPRP1
• Applications: Western Blot
• Purity: Purified (Highly Purified (~95%))
• Synonyms: IGFBP-7; Recombinant; Mouse; Western Blot Positive Control; Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 7; IBP-7; IGF-binding Protein 7; IBP7; GFBP-rP1; MAC25 Protein; PGI2-stimulating Factor; Prostacyclin-stimulating Factor; Tumor-derived Adhesion Factor; ; Insulin-like Growth Factor-binding Protein 7; TAF; IGFBP7; MAC25; PSF; IGFBP7 recombinant protein

• Purity/Purification: Purified (Highly Purified (~95%))
• Form/Format: Supplied as a liquid in SDS-PAGE sample buffer (reduced).

• Applicable Applications for IGFBP7 recombinant protein: Western Blot
• Application Notes: Suitable for use in Western Blot. Not suitable for use in ELISA.; Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
• Source Information: Recombinant mouse IGFBP-7 (28-281aa), expressed in Sf21 cells as human CD33-His tag at the N-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.; Shipping Temperature: Blue Ice

Related Product Information for IGFBP7 recombinant protein

Unlike insulin, both IGF-I and IGF-II circulate in plasma tightly bound to specific binding proteins. Two major forms of IGF-binding proteins have been identified in human plasma, a low molecular weight form and a high molecular weight form. The low molecular weight IGF-binding protein (IGFBP1) is synthesized in liver, secretory endometrium, and decidua. It binds both IGF I and IGF II with high affinity. Insulin-like growth factor binding protein-1 (IGFBP-1 ~30kD; also known as IBP1 or placental protein 12, or IGF-BP25; chromosome 7p14-p12)) is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high-affinity IGF binding proteins (IGFBP1-6) and at least four low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). The IGFBP members are cysteine- rich proteins with conserved cysteine residues, clustered in the amino-terminal and the carboxy-terminal regions of the molecule. Contained within IGFBP-1 and -2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF-independent action. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF-independent manner.

IGF-BP7 is expressed in a wide range of normal human tissues and it generally shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin. It plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. Additionally, IGF-BP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle, and increased apoptosis. Recombinant human IGF-BP7 is a 26.4kD protein consisting of 257aa residues. IGFBP-RP1 is closely related to CTGF (IGFBP-8), NOV (IGFBP-9) and CYR61 (IGFBP-10). The four proteins constitute a subfamily of low-affinity IGFBPs.

Product Categories/Family for IGFBP7 recombinant protein

Growth Factors; Cytokines; Growth Factors-IGF

NCBI and Uniprot Product Information

• NCBI GI #: 4504619
• NCBI GeneID: 3490
• NCBI Accession #: NP_001544.1
• NCBI GenBank Nucleotide #: NM_001553.1
• UniProt Accession #: Q16270; Q07822; Q53YE6; Q9UCA8
• Molecular Weight: ~35kD
• NCBI Official Full Name: insulin-like growth factor-binding protein 7
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 7
• NCBI Official Symbol: IGFBP7
• NCBI Official Synonym Symbols: AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; IGFBP-7v; IGFBPRP1
• NCBI Protein Information: insulin-like growth factor-binding protein 7; IGFBP-rP1; angiomodulin; OTTHUMP00000158974; IGF-binding protein 7; PGI2-stimulating factor; tumor-derived adhesion factor; prostacyclin-stimulating factor
• UniProt Protein Name: Insulin-like growth factor-binding protein 7
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP7
• UniProt Synonym Gene Names: MAC25; PSF
• UniProt Entry Name: IBP7_HUMAN