Rabbit anti-Mouse, Rat Tyrosine Hydroxylase Polyclonal Antibody | anti-TYH antibody

Tyrosine Hydroxylase (TYH, Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase) (APC)

Cat. Num. AAA6504288

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; Tyrosine Hydroxylase (TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase) (APC); anti-TYH antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the C-terminus of mouse Tyrosine Hydroxylase, MW ~58kD. Species Crossreactivity: Rat.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TYH antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:100; WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant Tyrosine Hydroxylase
• Conjugate: APC
• Positive Control: Rat Brain Lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry: 1:100 dilution of T9237-10L was used on rat (left and center) and mouse (right) brain (paraffin embedded tissue).)

Western Blot (WB)

(Western Blot Analysis: Rat Brain lysate was resolved by electrophoresis, transferred to PVDF and probed with T9237-10L (1:1000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Tyrosine Hydroxylase (~58kD).)

Product Categories/Family for anti-TYH antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 88900503
• NCBI GeneID: 7054
• NCBI Accession #: NP_000351.2
• NCBI GenBank Nucleotide #: NM_000360.3
• UniProt Accession #: P07101; Q15585; Q15588; Q15589; B7ZL70
• Molecular Weight: 58,600 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase isoform b
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Oxidoreductase; Endoplasmic reticulum; Mitochondrial; Vesicle; Amino Acid Metabolism - tyrosine; EC 1.14.16.2

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; eye photoreceptor cell development; organ morphogenesis; response to ethanol; circadian sleep/wake cycle; response to zinc ion; cerebral cortex development; response to activity; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to salt stress; response to estradiol stimulus; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; learning; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive