Leukemia Inhibitory Factor, Rat Active Protein | LIF active protein

Leukemia Inhibitory Factor, Recombinant, Rat (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI)

Cat. Num. AAA650372

• Gene Names: LIF; CDF; DIA; HILDA
• Purity: Highly Purified; 95% (SDS-PAGE). Endotoxin: 0.1ng/mg (LAL)
• Synonyms: Leukemia Inhibitory Factor; Rat; Recombinant; Rat (LIF; Cholinergic Differentiation Factor; CDF; DIA; Differentiation-stimulating Factor; D Factor; HILDA; Melanoma-derived LPL Inhibitor; MLPLI); LIF active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% (SDS-PAGE). Endotoxin: 0.1ng/mg (LAL)
• Form/Format: Supplied as a liquid in PBS, 0.02% Tween-20. Dilute further in PBS, 1% BSA or 0.02% Tween-20 to a final concentration 1ug/ml.

• Activity: The biological activity of recombinant human LIF is measured by its ability to stimulate the differentiation of the M1 mouse myeloid leukemic cell line. The specific activity is 1x10e8 units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1ml agar cultures.
• Preparation and Storage: May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in PBS, 1% BSA or 0.02% Tween 20.

Related Product Information for LIF active protein

Leukemia Inhibitory Factor (LIF) human, a 20kD protein containing 181aa residues, is a multifunctional gylcoprotein that induces macrophage differentiation and supresses the proliferation of the M1 myeloid cell line. Synonyms for LIF include: differentiation-inhibitory activity (DIA) for murine embryonic stem (ES) cells, human interleukin for DA cells (HLDA), hepatocyte stimulation factor III, cholinergic neuronal differentiation factor and lipoprotein lipase inhibitor. Leukemia Inhibitory Factor plays an important role, along with Interleukin-6 (IL-6) and granulocyte-colony stimulating factor (G-CSF), in the regulation of early hematopoietic stem cells. LIF is also important in the release of calcium from bone tissue. Human LIF is active on human and mouse cells, whereas mouse LIF is active on mouse cells. There is a 78% homology between mouse and human.

Product Categories/Family for LIF active protein

Growth Factors; Cytokines; Growth Factors-LIF

NCBI and Uniprot Product Information

• NCBI GI #: 178415
• NCBI GeneID: 3976
• UniProt Accession #: P15018; Q52LZ2; B2RCW7
• Molecular Weight: 19.7kD
• NCBI Official Full Name: leukemia inhibitory factor
• NCBI Official Synonym Full Names: leukemia inhibitory factor (cholinergic differentiation factor)
• NCBI Official Symbol: LIF
• NCBI Official Synonym Symbols: CDF; DIA; HILDA
• NCBI Protein Information: leukemia inhibitory factor; MLPLI; D factor; OTTHUMP00000198698; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation stimulating factor; differentiation-stimulating factor; differentiation inhibitory activity
• UniProt Protein Name: Leukemia inhibitory factor
• Protein Family: Leukemia inhibitory factor
• UniProt Gene Name: LIF
• UniProt Synonym Gene Names: HILDA
• UniProt Entry Name: LIF_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq]

Uniprot Description

Function: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Subcellular location: Secreted.

Pharmaceutical use: In phase II clinical trial. The drug is being developed by Amrad to assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART).

Sequence similarities: Belongs to the LIF/OSM family.