IGFBP5, Recombinant, Mouse, Western Blot Positive Control Recombinant Protein | Igfbp5 recombinant protein

IGFBP5, Recombinant, Mouse, Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 5, IBP-5, IGF-binding Protein 5, IGFBP-5, IBP5)

Cat. Num. AAA650300

• Gene Names: Igfbp5; IGF-BP5
• Applications: Western Blot
• Purity: Purified (Highly Purified (~95%))
• Synonyms: IGFBP5; Recombinant; Mouse; Western Blot Positive Control; Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 5; IBP-5; IGF-binding Protein 5; IGFBP-5; IBP5); Insulin-like Growth Factor-binding Protein 5; IBP5; Igfbp5 recombinant protein

• Purity/Purification: Purified (Highly Purified (~95%))
• Form/Format: Supplied as a liquid in SDS-PAGE sample buffer (reduced).

• Applicable Applications for Igfbp5 recombinant protein: Western Blot
• Application Notes: Suitable for use in Western Blot. Not suitable for use in ELISA.; Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
• Source Information: Recombinant mouse IGFBP5, expressed in NSO cells as CD33 fusion tag protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.; Shipping Temperature: Blue Ice

Related Product Information for Igfbp5 recombinant protein

The insulin like growth factors (IGFs) are the major growth- promoting factors in the plasma. IGFs are secreted by a variety of cells and exert a multitude of effects on cellular survival, growth and differentiation. The A and B domains of IGFs are identical to insulin. IGF initiates their biological action through binding to the type IGF receptor (IGF-1R), a heterotrimeric protein complex with a tyrosine kinase activity. The IGF-IIR lacks the kinase activity and is actually identical to the mannose- 6-phophate receptor. Unlike most other peptide hormones, IGFs are complexed with specific binding proteins in the plasma known IGF Binding proteins (IGFBPs). At least 6 related IGFBPs (IGFBP1-6) have been well characterized. Recently, IGFBP-7/Mac25/prostacyclin- stimulating factor (PSF)/tumor adhesion factor (TAF) was originally identified as a cDNA derived from leptomeninges. These proteins are present in plasma in high concentration as compared to the membrane IGFRs. Therefore, IGFBPs have the potential to modulate the IGF action. IGFBPs have been shown to either inhibit or stimulate the IGF effects. The primary structures of mammalian IGFBPs appear to contain three distinct domains of roughly equivalent sizes: the conserved N-terminal domain, the highly variable mid region, and the conserved C-terminal domain. Human IGFBPs share approximately 36% identity.

Human IGFBP-5 is a 252aa mature protein that is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21kD fragment.

Product Categories/Family for Igfbp5 recombinant protein

Growth Factors; Cytokines; Growth Factors-IGF

NCBI and Uniprot Product Information

• NCBI GI #: 6981088
• NCBI GeneID: 25285
• NCBI Accession #: NP_036949.1
• NCBI GenBank Nucleotide #: NM_012817.1
• UniProt Accession #: P24594
• Molecular Weight: ~28-38kD
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: Igfbp5
• NCBI Official Synonym Symbols: IGF-BP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp5
• UniProt Synonym Gene Names: Igfbp-5
• UniProt Entry Name: IBP5_RAT