IGFBP3, Recombinant, Mouse, Western Blot Positive Control Recombinant Protein | Igfbp3 recombinant protein
IGFBP3, Recombinant, Mouse, Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 3, IBP-3, IGF-binding Protein 3, IGFBP-3, IBP3)
Gene Names
Igfbp3; IGF-BP3; MGC156773
Applications
Western Blot
Purity
Purified (Highly Purified (~95%))
Synonyms
IGFBP3; Recombinant; Mouse; Western Blot Positive Control; Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 3; IBP-3; IGF-binding Protein 3; IGFBP-3; IBP3); Insulin-like Growth Factor-binding Protein 3; IBP3; Igfbp3 recombinant protein
Purity/Purification
Purified (Highly Purified (~95%))
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for Igfbp3 recombinant protein
Western Blot
Application Notes
Suitable for use in Western Blot. It is not suitable for ELISA or other applications where native protein is required.
Recommended Dilutions: Western Blot: 10 ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.
Recommended Dilutions: Western Blot: 10 ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.
Source Information
Recombinant mouse IGFBP3 (22-291aa), expressed as human CD33 signal peptide in NSO cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for Igfbp3 recombinant protein
The insulin like growth factors (IGFs) are the major growth- promoting factors in the plasma. IGFs are secreted by a variety of cells and exert a multitude of effects on cellular survival, growth and differentiation. The A and B domains of IGFs are identical to insulin. IGF initiates their biological action through binding to the type IGF receptor (IGF-1R), a heterotrimeric protein complex with a tyrosine kinase activity. The IGF-IIR lacks the kinase activity and is actually identical to the mannose- 6-phophate receptor. Unlike most other peptide hormones, IGFs are complexed with specific binding proteins in the plasma known IGF Binding proteins (IGFBPs). At least 6 related IGFBPs (IGFBP1-6) have been well characterized. Recently, IGFBP-7/Mac25/prostacyclin- stimulating factor (PSF)/tumor adhesion factor (TAF) was originally identified as a cDNA derived from leptomeninges. These proteins are present in plasma in high concentration as compared to the membrane IGFRs. Therefore, IGFBPs have the potential to modulate the IGF action. IGFBPs have been shown to either inhibit or stimulate the IGF effects. The primary structures of mammalian IGFBPs appear to contain three distinct domains of roughly equivalent sizes: the conserved N-terminal domain, the highly variable mid region, and the conserved C-terminal domain. Human IGFBPs share approximately 36% identity. Recently several groups of cysteine-rich proteins with discrete, but striking, structural and functional similarities to the IGFBPs. This has led to the proposal of an IGFBP superfamily, comprised of the IGFBPs and these IGFBP-related proteins (IGFBP-rP1-9).
Product Categories/Family for Igfbp3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~40-50D
NCBI Official Full Name
insulin-like growth factor-binding protein 3
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 3
NCBI Official Symbol
Igfbp3
NCBI Official Synonym Symbols
IGF-BP3; MGC156773
NCBI Protein Information
insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; insulin-like growth factor-binding protein (IGF-BP3)
UniProt Protein Name
Insulin-like growth factor-binding protein 3
Protein Family
UniProt Gene Name
Igfbp3
UniProt Synonym Gene Names
Igfbp-3
UniProt Entry Name
IBP3_RAT
Similar Products
Product Notes
The Igfbp3 igfbp3 (Catalog #AAA650208) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IGFBP3, Recombinant, Mouse, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot. Suitable for use in Western Blot. It is not suitable for ELISA or other applications where native protein is required. Recommended Dilutions: Western Blot: 10 ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. Researchers should empirically determine the suitability of the Igfbp3 igfbp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGFBP3, Recombinant, Mouse, Western Blot Positive Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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