Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control Recombinant Protein
Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 4, IBP-4, IGF-binding Protein 4, IGFBP-4, IBP4)
Applications
Western Blot
Purity
Purified (Highly Purified (~95%))
Synonyms
Insulin Binding Protein 4; Recombinant; Human; Western Blot Positive Control; Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 4; IBP-4; IGF-binding Protein 4; IGFBP-4; IBP4); Insulin-like Growth Factor-binding Protein 4; IBP4; Western Blot Positive Control recombinant protein
Host
NSO Cells
Purity/Purification
Purified (Highly Purified (~95%))
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control recombinant protein
Western Blot
Application Notes
Suitable for use in Western Blot. Not suitable for use in ELISA.
Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
Source Information
Recombinant human IGFBP4 (mature protein 22-258aa) was expressed in NSO cells with a CD33 signal peptide.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control recombinant protein
The insulin like growth factors (IGFs) are the major growth- promoting factors in the plasma. IGFs are secreted by a variety of cells and exert a multitude of effects on cellular survival, growth and differentiation. The A and B domains of IGFs are identical to insulin. IGF initiates their biological action through binding to the type IGF receptor (IGF-1R), a heterotrimeric protein complex with a tyrosine kinase activity. The IGF-IIR lacks the kinase activity and is actually identical to the mannose- 6-phophate receptor. Unlike most other peptide hormones, IGFs are complexed with specific binding proteins in the plasma known IGF Binding proteins (IGFBPs). At least 6 related IGFBPs (IGFBP1-6) have been well characterized. Recently, IGFBP-7/Mac25/prostacyclin- stimulating factor (PSF)/tumor adhesion factor (TAF) was originally identified as a cDNA derived from leptomeninges. These proteins are present in plasma in high concentration as compared to the membrane IGFRs. Therefore, IGFBPs have the potential to modulate the IGF action. IGFBPs have been shown to either inhibit or stimulate the IGF effects. The primary structures of mammalian IGFBPs appear to contain three distinct domains of roughly equivalent sizes: the conserved N-terminal domain, the highly variable mid region, and the conserved C-terminal domain. Human IGFBPs share approximately 36% identity.
Insulin-like growth factor binding protein-4 (IGFBP-4, 22-237aa mature protein, protein accession # P22692) is expressed in multiple tissues including adrenal, testis, spleen, heart, lung, kidney, liver, stomach, hypothalamus, and brain cortex. High expression levels are found in the non-paranchymal cells of the liver.4 IGFBP-4 is the predominant IGFBP expressed by human osteoblast-like cells and is also expressed in skin fibroblasts.
Insulin-like growth factor binding protein-4 (IGFBP-4, 22-237aa mature protein, protein accession # P22692) is expressed in multiple tissues including adrenal, testis, spleen, heart, lung, kidney, liver, stomach, hypothalamus, and brain cortex. High expression levels are found in the non-paranchymal cells of the liver.4 IGFBP-4 is the predominant IGFBP expressed by human osteoblast-like cells and is also expressed in skin fibroblasts.
Product Categories/Family for Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control recombinant protein
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Product Notes
The Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control (Catalog #AAA650189) is a Recombinant Protein produced from NSO Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot. Suitable for use in Western Blot. Not suitable for use in ELISA. Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels. Researchers should empirically determine the suitability of the Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Binding Protein 4, Recombinant, Human, Western Blot Positive Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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