Fibroblast Growth Factor, Acidic Active Protein | FGF1 active protein

Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

Cat. Num. AAA650085

• Gene Names: FGF1; ECGF; FGF-1
• Purity: Highly Purified; 97% as determined by: RP-HPLC and SDS-PAGE. Chromatographically purified.
• Synonyms: Fibroblast Growth Factor; Acidic; Recombinant; Human (FGFa); FGF1 active protein

• Purity/Purification: Highly Purified; 97% as determined by: RP-HPLC and SDS-PAGE. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O to a concentration of 0.1-0.25mg/ml. Allow sample to sit for 5 minutes at 4ºC, spin to remove precipitant.
• Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

• Biological Activity: The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <1ng/ml.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for FGF1 active protein

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Recombinant Human Fibroblast Growth Factor-acidic (FGF-1) is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15,967 Daltons.

Product Categories/Family for FGF1 active protein

Growth Factors; Cytokines; Growth Factors-FGF

NCBI and Uniprot Product Information

• NCBI GI #: 27806633
• NCBI GeneID: 281160
• NCBI Accession #: NP_776480.1
• NCBI GenBank Nucleotide #: NM_174055.2
• UniProt Accession #: P03968; Q3ZBL8
• Molecular Weight: 15.967kD
• NCBI Official Full Name: fibroblast growth factor 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1 (acidic)
• NCBI Official Symbol: FGF1
• NCBI Official Synonym Symbols: ECGF; FGF-1
• NCBI Protein Information: fibroblast growth factor 1; aFGF; HBGF-1; EDGF II; prostatropin; endothelial growth factor; acidic fibroblast growth factor; heparin-binding growth factor 1; fibroblast growth factor, acidic; acidic eye-derived growth factor II; endothelial cell growth factor beta and alpha chains
• UniProt Protein Name: Fibroblast growth factor 1
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF1
• UniProt Synonym Gene Names: AFGF; FGFA; HBGF-1; FGF-1; EDGF II; aFGF; ECGF; HBGF-1
• UniProt Entry Name: FGF1_BOVIN

Uniprot Description

Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Ref.11 Ref.12

Subunit structure: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu2+-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with S100A13. Interacts with FGFBP1

By similarity. Interacts with LRRC59

By similarity. Interacts with CSNKA, CSNKB and FIBP

By similarity. While binding with LRRC59, CSNKA and FIBP seem mutually exclusive, CSNKB and FIBP may cooperatively interact with FGF1

By similarity. Interacts with SYT1. Ref.11

Subcellular location: Secreted. Cytoplasm

By similarity. Cytoplasm › cell cortex

By similarity. Cytoplasm › cytosol. Nucleus. Note: Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu2+ ions and S100A13. Secreted in a complex with SYT1. Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59

By similarity. Ref.11 Ref.12

Post-translational modification: In the nucleus, phosphorylated by PKC/PRKCD

By similarity.

Sequence similarities: Belongs to the heparin-binding growth factors family.