Western Blot (WB)
(The anti-STK16 Pab is used in Western blot to detect STK16 in mouse brain tissue lysate.)
Rabbit anti-Human, Mouse STK16 Polyclonal Antibody | anti-STK16 antibody
STK16, NT (Serine/Threonine-protein Kinase 16, hPSK, MPSK, MPSK1, Myristoylated And Palmitoylated Serine/Threonine-protein Kinase, PKL12, Protein Kinase PKL12, TGF-beta-stimulated Factor 1, TSF1, TSF-1) (APC)
Gene Names
STK16; KRCT; MPSK; TSF1; PKL12
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
STK16; Polyclonal Antibody; NT (Serine/Threonine-protein Kinase 16; hPSK; MPSK; MPSK1; Myristoylated And Palmitoylated Serine/Threonine-protein Kinase; PKL12; Protein Kinase PKL12; TGF-beta-stimulated Factor 1; TSF1; TSF-1) (APC); anti-STK16 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human STK16. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-STK16 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human STK16 (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-STK16 Pab is used in Western blot to detect STK16 in mouse brain tissue lysate.)
Western Blot (WB)
(The anti-STK16 Pab is used in Western blot to detect STK16 in mouse brain tissue lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(Western blot analysis of STK16 (arrow) using rabbit polyclonal STK16 Antibody (S32). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STK16 gene.)
Western Blot (WB)
(Western blot analysis of STK16 (arrow) using rabbit polyclonal STK16 Antibody (S32). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STK16 gene.)
Product Categories/Family for anti-STK16 antibody
References
1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Berson, A.E., et al., Biochem. Biophys. Res. Commun. 259(3):533-538 (1999). 3. Ligos, J.M., et al., Biochem. Biophys. Res. Commun. 249(2):380-384 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,656 Da
NCBI Official Full Name
serine/threonine-protein kinase 16
NCBI Official Synonym Full Names
serine/threonine kinase 16
NCBI Official Symbol
STK16
NCBI Official Synonym Symbols
KRCT; MPSK; TSF1; PKL12
NCBI Protein Information
serine/threonine-protein kinase 16; TGF-beta-stimulated factor 1; TSF-1; hPSK; myristoylated and palmitoylated serine/threonine-protein kinase; protein kinase PKL12; protein kinase expressed in day 12 fetal liver; tyrosine-protein kinase STK16
UniProt Protein Name
Serine/threonine-protein kinase 16
Protein Family
UniProt Gene Name
STK16
UniProt Synonym Gene Names
MPSK1; PKL12; TSF1; MPSK; TSF-1
UniProt Entry Name
STK16_HUMAN
Uniprot Description
MPSK1: a protein kinase of the STE20 family. Activated by caspase-mediated cleavage
Protein type: EC 2.7.10.2; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; NAK family
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; Golgi-associated vesicle
Molecular Function: protein serine/threonine kinase activity; protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; regulation of signal transduction; positive regulation of transcription from RNA polymerase II promoter
Research Articles on STK16
Similar Products
Product Notes
The STK16 stk16 (Catalog #AAA6500129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STK16, NT (Serine/Threonine-protein Kinase 16, hPSK, MPSK, MPSK1, Myristoylated And Palmitoylated Serine/Threonine-protein Kinase, PKL12, Protein Kinase PKL12, TGF-beta-stimulated Factor 1, TSF1, TSF-1) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STK16 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the STK16 stk16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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