Rabbit anti-Human SCF Polyclonal Antibody | anti-SCF antibody

SCF, CT (Kit Ligand, C-kit Ligand, Stem Cell Factor, Mast Cell Growth Factor, MGF, KITLG) (Biotin)

Cat. Num. AAA6498532

• Gene Names: KITLG; SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SCF; Polyclonal Antibody; CT (Kit Ligand; C-kit Ligand; Stem Cell Factor; Mast Cell Growth Factor; MGF; KITLG) (Biotin); anti-SCF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KITLG.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SCF antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: ELISA: 1:1,000; WB: 1:50-1:100; FC/FACS: 1:10-1:50; IF: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human KITLG (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KITLG Antibody (C-term) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of KITLG (arrow) using rabbit polyclonal KITLG Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).)

Immunofluorescence (IF)

(Immunofluorescence analysis of anti-KITLG Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SCF antibody

Antibodies; Growth Factors; Cytokines

References

1. Young, S.M., Cell. Signal. 19(12), 2572-2581 (2007). 2. Pick, M., Stem Cells 25(9), 2206-2214 (2007). 3. Yasuda, A., Dig. Dis. Sci. 52(9), 2292-2300 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4505175
• NCBI GeneID: 4254
• NCBI Accession #: NP_000890
• NCBI GenBank Nucleotide #: NM_000899.4
• UniProt Accession #: P21583; Q16487; Q68DZ2; Q7M4N8; Q9UQK7; A0AV09; A8K2Q4; B7ZLM4
• Molecular Weight: 18.5 kDa (165 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: kit ligand isoform b
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• Protein Family: SCF-associated factor
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]