Western Blot (WB)
(Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649678. This demonstrates MBS649678 detected the ARHGAP24 protein (arrow).)
Rabbit anti-Human ARHGAP24 Polyclonal Antibody | anti-ARHGAP24 antibody
ARHGAP24, CT (ARHGAP24, FILGAP, Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72kD, Rho-type GTPase-activating protein 24, RhoGAP of 73kD, Sarcoma antigen NY-SAR-88, p73RhoGAP)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649678. This demonstrates MBS649678 detected the ARHGAP24 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649678. This demonstrates MBS649678 detected the ARHGAP24 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
ARHGAPs, such as ARHGAP24, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. Ref.6 Ref.7 Ref.8
Subunit structure: Interacts with FLNA. Ref.8
Subcellular location: Cytoplasm › cytoskeleton. Cell junction › adherens junction. Cell junction › focal adhesion. Cell projection. Note: Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions. Ref.7 Ref.8
Tissue specificity: Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells. Ref.6 Ref.7 Ref.8
Induction: Isoform 2 is up-regulated during capillary tube formation in umbilical vein endothelial cells. Ref.6
Domain: The coiled coil domain mediates the interaction with FLNA leading to its recruitment to lamellae.
Post-translational modification: Phosphorylated by ROCK, leading to activate the RacGAP activity. Ref.8
Sequence similarities: Contains 1 PH domain.Contains 1 Rho-GAP domain.
Sequence caution: The sequence AAO65178.1 differs from that shown. Reason: Frameshift at several positions.
Research Articles on ARHGAP24
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Product Notes
The ARHGAP24 arhgap24 (Catalog #AAA649678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP24, CT (ARHGAP24, FILGAP, Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72kD, Rho-type GTPase-activating protein 24, RhoGAP of 73kD, Sarcoma antigen NY-SAR-88, p73RhoGAP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP24 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the ARHGAP24 arhgap24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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