Western Blot (WB)
(Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35ug/lane). PPM1F(arrow) was detected using the purified Pab.)
Rabbit anti-Human PPM1F Polyclonal Antibody | anti-PPM1F antibody
PPM1F, NT (Protein Phosphatase 1F, Ca(2+)/Calmodulin-dependent Protein Kinase Phosphatase, CaM-kinase Phosphatase, CaMKPase, Partner of PIX 2, Protein Fem-2 Homolog, hFEM-2, KIAA0015, POPX2) (APC)
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35ug/lane). PPM1F(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-PPM1F Pab in mouse liver tissue lysate (35ug/lane). PPM1F(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PPM1F (arrow) using PPM1F Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
PPM1F: a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members can be negative regulators of cell stress response pathways. Interact with PAK-interacting exchange factor beta (ARHGEF7), blocking the effects PAK1, a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CaMK2-gamma) is one of the substrates of this phosphatase. Promotes apoptosis.
Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, dual-specificity
Chromosomal Location of Human Ortholog: 22q11.22
Cellular Component: protein complex; perinuclear region of cytoplasm; cytosol
Molecular Function: protein binding; calmodulin-dependent protein phosphatase activity; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity
Biological Process: histone dephosphorylation; positive regulation of focal adhesion formation; positive regulation of chemotaxis; positive regulation of stress fiber formation; negative regulation of peptidyl-serine phosphorylation; positive regulation of caspase activity; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of growth
Research Articles on PPM1F
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Product Notes
The PPM1F ppm1f (Catalog #AAA6495478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPM1F, NT (Protein Phosphatase 1F, Ca(2+)/Calmodulin-dependent Protein Kinase Phosphatase, CaM-kinase Phosphatase, CaMKPase, Partner of PIX 2, Protein Fem-2 Homolog, hFEM-2, KIAA0015, POPX2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1F can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PPM1F ppm1f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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