Rabbit anti-Human PKC theta Polyclonal Antibody | anti-PRKCT antibody

PKC theta, CT (Protein Kinase C theta Type, nPKC-theta, PRKCQ, PRKCT) (PE)

Cat. Num. AAA6495234

• Gene Names: PRKCQ; PRKCT; nPKC-theta
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PKC theta; Polyclonal Antibody; CT (Protein Kinase C theta Type; nPKC-theta; PRKCQ; PRKCT) (PE); anti-PRKCT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKC theta.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PRKCT antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 318-348 in the C-terminal region of human PKC theta, UniProt Accession #Q04759.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6000749 in placenta lysate. PKC theta (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS6000749, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Product Categories/Family for anti-PRKCT antibody

Antibodies; Protein Kinases

References

1. Carrasco, S., et al., Mol. Biol. Cell 15(6):2932-2942 (2004). 2. Sun, L., et al., Blood 103(3):948-954 (2004). 3. Burchfield, J.G., et al., J. Biol. Chem. 279(18):18623-18632 (2004). 4. Ishaq, M., et al., J. Biol. Chem. 278(41):39296-39302 (2003). 5. Cipriani, B., et al., J. Immunol. 169(10):5761-5770 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 334848126
• NCBI GeneID: 5588
• NCBI Accession #: NP_001229342.1
• NCBI GenBank Nucleotide #: NM_001242413.1
• UniProt Accession #: Q04759; Q14DH6; Q3MJF1; Q64FY5; Q9H508; Q9H549
• Molecular Weight: 81,865 Da
• NCBI Official Full Name: protein kinase C theta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C, theta
• NCBI Official Symbol: PRKCQ
• NCBI Official Synonym Symbols: PRKCT; nPKC-theta
• NCBI Protein Information: protein kinase C theta type
• UniProt Protein Name: Protein kinase C theta type
• UniProt Gene Name: PRKCQ
• UniProt Synonym Gene Names: PRKCT
• UniProt Entry Name: KPCT_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCT: an AGC kinase of the PKC family. A novel PKC: Ca2+ independent but still regulated by PS, DAG and phorbol esters. Contains 2 zinc-dependent phorbol-ester and DAG binding domains. Expressed in skeletal muscle and hematopoietic cells. May play a role in T cell receptor signaling and insulin resistance. Required for TCR-induced NF-kappaB activation in mature T lymphocytes.

Protein type: Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; immunological synapse; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ubiquitin-protein ligase activity; ATP binding

Biological Process: positive regulation of interleukin-17 production; axon guidance; phototransduction, visible light; platelet activation; apoptosis; positive regulation of interleukin-2 biosynthetic process; membrane protein ectodomain proteolysis; negative regulation of insulin receptor signaling pathway; protein ubiquitination; protein amino acid phosphorylation; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; rhodopsin mediated signaling; positive regulation of interleukin-4 production; regulation of rhodopsin mediated signaling; regulation of transcription, DNA-dependent; innate immune response; positive regulation of T cell proliferation; regulation of cell growth; positive regulation of T cell activation; inflammatory response; blood coagulation; cell structure disassembly during apoptosis