Rabbit anti-Human PSMD11 Polyclonal Antibody | anti-PSMD11 antibody

PSMD11, CT (26S Proteasome Non-ATPase Regulatory Subunit 11, 26S Proteasome Regulatory Subunit S9, 26S Proteasome Regulatory Subunit RPN6, 26S Proteasome Regulatory Subunit p44.5) (MaxLight 490)

Cat. Num. AAA6494993

• Gene Names: PSMD11; S9; Rpn6; p44.5
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PSMD11; Polyclonal Antibody; CT (26S Proteasome Non-ATPase Regulatory Subunit 11; 26S Proteasome Regulatory Subunit S9; 26S Proteasome Regulatory Subunit RPN6; 26S Proteasome Regulatory Subunit p44.5) (MaxLight 490); anti-PSMD11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMD11.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-PSMD11 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PSMD11 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PSMD11 Antibody (C-term) in 293, Jurkat cell line lysates (35ug/lane). PSMD11 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PSMD11 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(PSMD11 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PSMD11 antibody detected the PSMD11 protein (arrow).)

Product Categories/Family for anti-PSMD11 antibody

Antibodies; Proteasomes

References

1. Saito, A., Gene 203(2), 241-250 (1997). 2. Hoffman, L., FEBS Lett. 404(2-3), 179-184 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 28872725
• NCBI GeneID: 5717
• NCBI Accession #: NP_002806
• NCBI GenBank Nucleotide #: NM_002815.3
• UniProt Accession #: O00231; O00495; Q53FT5; A8K3I7; E1P663
• Molecular Weight: 47,535 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 11
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 11
• NCBI Official Symbol: PSMD11
• NCBI Official Synonym Symbols: S9; Rpn6; p44.5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 11
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 11
• UniProt Gene Name: PSMD11
• UniProt Entry Name: PSD11_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMD11: Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Belongs to the proteasome subunit S9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; proteasome complex; membrane; proteasome regulatory particle; cytosol; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; stem cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; proteasome assembly; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle