Rabbit anti-Human PARK2 Polyclonal Antibody | anti-PARK2 antibody

PARK2, NT (E3 Ubiquitin-protein Ligase Parkin, PRKN, Parkinson Juvenile Disease Protein 2, Parkinson Disease Protein 2) (HRP)

Cat. Num. AAA6492097

• Gene Names: PARK2; PDJ; PRKN; AR-JP; LPRS2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PARK2; Polyclonal Antibody; NT (E3 Ubiquitin-protein Ligase Parkin; PRKN; Parkinson Juvenile Disease Protein 2; Parkinson Disease Protein 2) (HRP); anti-PARK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Park2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PARK2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide in the C-terminal region of human Park2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of anti-PARK2 (Parkin) Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). PARK2 (Parkin)(arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Parkin Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Parkin Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(Parkin Antibody (N-term) (AP6402a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parkin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-PARK2 antibody

Antibodies; Ubiquitin Ligases

References

1. Kumru, H., et al., Ann. Neurol. 56(4):599-603 (2004). 2. Pigullo, S., et al., Parkinsonism Relat. Disord. 10(6):357-362 (2004). 3. Yao, D., et al., Proc. Natl. Acad. Sci. USA 101(29):10810-10814 (2004). 4. West, A.B., et al., J. Biol. Chem. 279(28):28896-28902 (2004). 5. Wang, F., et al., Genes Chromosomes Cancer 40(2):85-96 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4758884
• NCBI GeneID: 5071
• NCBI Accession #: NP_004553.1; NP_054642.1; NP_054643.1
• NCBI GenBank Nucleotide #: NM_004562.1
• Molecular Weight: 46,413 Da
• NCBI Official Full Name: parkin isoform 1
• NCBI Official Synonym Full Names: parkin RBR E3 ubiquitin protein ligase
• NCBI Official Symbol: PARK2
• NCBI Official Synonym Symbols: PDJ; PRKN; AR-JP; LPRS2
• NCBI Protein Information: E3 ubiquitin-protein ligase parkin; parkinson juvenile disease protein 2; parkinson protein 2, E3 ubiquitin protein ligase (parkin); Parkinson disease (autosomal recessive, juvenile) 2, parkin
• Protein Family: E3 ubiquitin-protein ligase