Rabbit anti-Human PAPSS1 Polyclonal Antibody | anti-PAPSS1 antibody

PAPSS1, CT (A607) (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate Synthethase 1, PAPS Synthethase 1, Sulfurylase Kinase 1, SK1, ATPSK1, PAPSS) (PE)

Cat. Num. AAA6491965

• Gene Names: PAPSS1; SK1; PAPSS; ATPSK1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PAPSS1; Polyclonal Antibody; CT (A607) (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate Synthethase 1; PAPS Synthethase 1; Sulfurylase Kinase 1; SK1; ATPSK1; PAPSS) (PE); anti-PAPSS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PAPSS1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PAPSS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PAPSS1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PAPSS1 Antibody (C-term A607) in A2058 cell line lysates (35ug/lane). PAPSS1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PAPSS1 antibody

Antibodies; Enzymes

References

1. Biochemistry 43(14), 4356-4365 (2004). 2. IUBMB Life 55(1), 1-11 (2003). 3. Biochem. J. 365(PT 2), 497-504 (2002). 4. Biochem. Biophys. Res. Commun. 268(2), 437-444 (2000). 5. FASEB J. 14(2), 345-354 (2000). 6.N Bergelin, et al. Endocrinology Vol. 150, No. 5 2055-2063. (Applications: WB)(E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 46094058
• NCBI GeneID: 9061
• NCBI Accession #: NP_005434
• NCBI GenBank Nucleotide #: NM_005443.4
• UniProt Accession #: O43252; O43841; O75332; Q6IAX6; Q96FB1; Q96TF4; Q9P1P9; Q9UE98
• Molecular Weight: 70.9 kDa (626aa)
• NCBI Official Full Name: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Synonym Full Names: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Symbol: PAPSS1
• NCBI Official Synonym Symbols: SK1; PAPSS; ATPSK1
• NCBI Protein Information: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• UniProt Protein Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• Protein Family: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
• UniProt Gene Name: PAPSS1
• UniProt Synonym Gene Names: ATPSK1; PAPSS; PAPS synthase 1; PAPSS 1; SK 1; SK1; SAT
• UniProt Entry Name: PAPS1_HUMAN

NCBI Description

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]

Uniprot Description

PAPSS1: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Protein type: Kinase, other; Other Amino Acids Metabolism - selenoamino acid; Nucleotide Metabolism - purine; EC 2.7.1.25; Energy Metabolism - sulfur; EC 2.7.7.4

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: cytosol

Molecular Function: adenylylsulfate kinase activity; ATP binding; sulfate adenylyltransferase (ATP) activity; nucleotidyltransferase activity

Biological Process: sulfate assimilation; glycosaminoglycan metabolic process; xenobiotic metabolic process; carbohydrate metabolic process; pathogenesis; phosphorylation; skeletal development; 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process