Rabbit anti-Human OPRS1 Polyclonal Antibody | anti-OPRS1 antibody

OPRS1, NT (Sigma Non-opioid Intracellular Receptor 1, Sigma 1-type Opioid Receptor, Sigma1-receptor, Sigma1R, SIG-1R, hSigmaR1, SR31747-binding Protein, SR-BP, Aging-associated Gene 8 Protein, OPRS1) (PE)

Cat. Num. AAA6491100

• Gene Names: SIGMAR1; SRBP; ALS16; OPRS1; SR-BP; SIG-1R; SR-BP1; sigma1R; hSigmaR1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: OPRS1; Polyclonal Antibody; NT (Sigma Non-opioid Intracellular Receptor 1; Sigma 1-type Opioid Receptor; Sigma1-receptor; Sigma1R; SIG-1R; hSigmaR1; SR31747-binding Protein; SR-BP; Aging-associated Gene 8 Protein; OPRS1) (PE); anti-OPRS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OPRS1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-OPRS1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human OPRS1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of OPRS1 (arrow) using rabbit polyclonal OPRS1 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OPRS1 gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using OPRS1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-OPRS1 antibody

Antibodies; Opioid Receptors; Agonists

References

1. Cobos, E.J., J. Neurochem. 102(3), 812-825 (2007). 2. Maurice, T., Pharmacol. Biochem. Behav. 84(4), 581-597 (2006). 3. Lee, I.T., Eur. J. Pharmacol. 578(2-3), 123-136 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5032117
• NCBI GeneID: 10280
• NCBI Accession #: NP_005857
• NCBI GenBank Nucleotide #: NM_005866
• UniProt Accession #: Q99720; O00673; O00725; Q0Z9W6; Q153Z1; Q2TSD1; Q53GN2; Q7Z653; Q8N7H3; Q9NYX0; D3DRM7
• Molecular Weight: 21,013 Da
• NCBI Official Full Name: sigma non-opioid intracellular receptor 1 isoform 1
• NCBI Official Synonym Full Names: sigma non-opioid intracellular receptor 1
• NCBI Official Symbol: SIGMAR1
• NCBI Official Synonym Symbols: SRBP; ALS16; OPRS1; SR-BP; SIG-1R; SR-BP1; sigma1R; hSigmaR1
• NCBI Protein Information: sigma non-opioid intracellular receptor 1; SR31747 binding protein 1; aging-associated gene 8 protein; sigma 1-type opioid receptor
• UniProt Protein Name: Sigma non-opioid intracellular receptor 1
• UniProt Gene Name: SIGMAR1
• UniProt Synonym Gene Names: OPRS1; SRBP; SR-BP; SIG-1R; Sigma1-receptor; Sigma1R; hSigmaR1
• UniProt Entry Name: SGMR1_HUMAN

Uniprot Description

SIGMAR1: Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration. Defects in SIGMAR1 are the cause of amyotrophic lateral sclerosis type 16, juvenile (ALS16). ALS16 is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5- 10% of the cases. Belongs to the ERG2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear envelope; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; growth cone; integral to plasma membrane; integral to membrane; nuclear inner membrane; nuclear envelope; lipid particle; cell junction

Molecular Function: opioid receptor activity; drug binding

Biological Process: nervous system development; regulation of neuron apoptosis; lipid transport

Disease: Amyotrophic Lateral Sclerosis 16, Juvenile