Rabbit anti-Human, Mouse NAK Polyclonal Antibody | anti-NAK antibody

NAK (S172) (TBK, Serine/Threonine-protein Kinase TBK1, TANK-binding Kinase 1, T2K, NF-kappa-B-activating Kinase, TBK1) (FITC)

Cat. Num. AAA6489276

• Gene Names: TBK1; NAK; T2K
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: NAK; Polyclonal Antibody; NAK (S172) (TBK; Serine/Threonine-protein Kinase TBK1; TANK-binding Kinase 1; T2K; NF-kappa-B-activating Kinase; TBK1) (FITC); anti-NAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NAK. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NAK antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 150-181 of human NAK. Species sequence homology: Xenopus, zebrafish
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using N0017-97C. TBK1(arrow) was detected using N0017-97C.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human testis tissue reacted with N0017-97C, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of N0017-97C for immunohistochemistry.)

Product Categories/Family for anti-NAK antibody

Antibodies; Protein Kinases

References

1. Deng, W., J. Biol. Chem. 283(51), 35590-35597 (2008). 2. Chessler, A.D., J. Immunol. 181(11), 7917-7924 (2008). 3. Soulat, D., EMBO J. 27(15), 2135-2146 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 7019547
• NCBI GeneID: 29110
• NCBI Accession #: NP_037386
• NCBI GenBank Nucleotide #: NM_013254.3
• UniProt Accession #: Q9UHD2; Q8IYV3; Q9NUJ5; A8K4S4
• Molecular Weight: 84 kDa
• NCBI Official Full Name: serine/threonine-protein kinase TBK1
• NCBI Official Synonym Full Names: TANK-binding kinase 1
• NCBI Official Symbol: TBK1
• NCBI Official Synonym Symbols: NAK; T2K
• NCBI Protein Information: serine/threonine-protein kinase TBK1; NF-kB-activating kinase; NF-kappa-B-activating kinase
• UniProt Protein Name: Serine/threonine-protein kinase TBK1
• Protein Family: Probable serine/threonine-protein kinase
• UniProt Gene Name: TBK1
• UniProt Synonym Gene Names: NAK
• UniProt Entry Name: TBK1_HUMAN

NCBI Description

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]