Rabbit anti-Human, Mouse MYPT1 Polyclonal Antibody | anti-MYPT1 antibody

MYPT1, Phosphorylated (Thr696) (Myosin Phosphatase 1) (AP)

Cat. Num. AAA6489207

• Gene Names: PPP1R12A; MBS; M130; MYPT1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MYPT1; Polyclonal Antibody; Phosphorylated (Thr696) (Myosin Phosphatase 1) (AP); anti-MYPT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MYPT1 phosphorylated at Thr696 at ~130kD. Species Crossreactivity: mouse. Species sequence homology: primate, canine, rat, Xenopus, equine, chicken, bovine, monkey, Danio.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-MYPT1 antibody: Western Blot (WB)
• Application Notes: WB: 0.1ug/ml detects MYPT1 phosphorylated at Thr969 on 10ug of Calyculin/Okadaic treated and untreated HEK293 cell lysate.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human phosphorylated Thr696 of MYPT1 (KLH).
• Conjugate: AP
• Positive Control: Calyculin/Okadaic treated and untreated HEK293 cell lysates.
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot: HEK293 +/-Calyculin/Okadaic acid lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS622031 (1:5,000) dilution. Proteins were visualized using a Dnk anti-Rbt IgG:HRP conjugated to HRP and visualized using a chemiluminescence detection system. Arrow indicates Phospho-MYPT1 (Thr696) (~130kD).)

Product Categories/Family for anti-MYPT1 antibody

Antibodies; Myosin

NCBI and Uniprot Product Information

• NCBI GI #: 219842212
• NCBI GeneID: 4659
• NCBI Accession #: NP_001137357.1
• NCBI GenBank Nucleotide #: NM_001143885.1
• UniProt Accession #: O14974; Q2NKL4; Q569H0; Q86WU3; Q8NFR6; Q9BYH0; B4DZ09; F8VWB4
• Molecular Weight: 115 kDa
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 12A isoform a
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 12A
• NCBI Official Symbol: PPP1R12A
• NCBI Official Synonym Symbols: MBS; M130; MYPT1
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 12A; myosin binding subunit; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase myosin-binding subunit; protein phosphatase 1, regulatory (inhibitor) subunit 12A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 12A
• UniProt Gene Name: PPP1R12A
• UniProt Synonym Gene Names: MBS; MYPT1; Myosin phosphatase target subunit 1
• UniProt Entry Name: MYPT1_HUMAN

NCBI Description

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q15-q21

Cellular Component: kinetochore; nucleoplasm; centrosome; focal adhesion; contractile fiber; cytoplasm; actin cytoskeleton

Molecular Function: enzyme inhibitor activity; protein binding; signal transducer activity; phosphatase regulator activity; protein kinase binding

Biological Process: regulation of cell adhesion; mitosis; centrosome organization and biogenesis; regulation of nucleocytoplasmic transport; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; signal transduction; G2/M transition of mitotic cell cycle; protein amino acid dephosphorylation